摘要
为探索花蕾型灰毡毛忍冬花冠不开裂、花蕾期长等特性背后的分子机制,本研究以花蕾型品种‘龙花’和普通型品种‘白云’为实验材料,利用RNA-Seq技术对灰毡毛忍冬花冠开裂过程3个不同时期的花进行转录组比较分析。结果显示,各样品的Clean reads介于18685062~28236097条之间,拼接共得到146481个Unigenes,N50为1415 bp,平均长度为952 bp,其中66449个Unigenes得到功能注释,占Unigene总数的45.36%。相比于‘龙花’‘,白云’共有5525个Unigenes在花冠开裂初期差异表达;9559个Unigenes在中期差异表达,13420个Unigenes在末期差异表达。对上述差异基因进行GO分析,分别有37个、58个、59个GO类别在‘白云’花冠开裂过程初期、中期和末期被显著富集。差异基因KEGG分析显示分别有7个、5个和1个代谢通路在‘白云’花冠开裂初期、中期和末期显著富集,主要涉及淀粉和蔗糖的代谢、植物激素信号转导、苯丙素生物合成、核糖体等代谢通路。此外,还在转录组数据中挖掘得到13个MADS-box基因在两个品种间的花冠开裂过程中有差异表达。本研究可为后续灰毡毛忍冬花冠开裂关键基因的挖掘、克隆与功能分析提供参考,为探明灰毡毛忍冬花冠开裂的分子机理以及灰毡毛忍冬优良新品种的分子遗传育种提供科学依据。
In order to explore the molecular mechanism behind the characteristics of bud-type Lonicera macranthoides,such as no corolla dehiscence and long flowering period,we carried out a comparative analysis upon the transcriptome of L.macranthoides flowers at three different stages of corolla dehiscence process using RNA-Seq in this study,taking bud-type’Longhua’and common-type’Baiyun’as experimental materials.The results showed that the Clean reads of each sample ranged from 18685062 to 28236097.A total of 146481 Unigenes were obtained by assembly,N50 was 1415 bp,and the average length was 952 bp,among which,66449 Unigenes were annotated,accounting for 45.36%of the total.Compared with’Longhua’,5525 Unigenes were differentially expressed at the early stage of corolla dehiscence;9559 Unigenes were differentially expressed in the middle stage;and 13420 Unigenes were differentially expressed at the late stage.Go analysis showed that there were 37,58 and 59 GO classes significantly enrichedin the early,middle and late stages of corolla dehiscence,respectively.KEGG analysis showed that there were 7,5 and 1 metabolic pathways significantly enriched in the early,middle and late stages of corolla dehiscence respectively,mainly involving starch and sucrose metabolism,plant hormone signal transduction,phenylpropanoid biosynthesis,ribosome and other metabolic pathways.In addition,it is also found from transcriptome data that 13 MADS-box genes were differentially expressed during corolla dehiscence between the two cultivars.This study can provide a reference for further research,cloning and functional analysis of key genes for corolla dehiscence of L.macranthoides,and lay a foundation for research of molecular mechanism of L.macranthoides corolla dehiscence and molecular genetic breeding of new varieties of L.macranthoides.
作者
刘思思
乔中全
刘新民
王晓明
曾慧杰
李永欣
蔡能
Liu Sisi;Qiao Zhongquan;Liu Xinmin;Wang Xiaoming;Zeng Huijie;Li Yongxin;Cai Neng(College of Integrated Traditional Chinese and Westem Medicine,Hunan University of Chinese Medicine,Changsha,410208;Changsha Engineering Technology Research Center of Woody flower,Hunan Academy of Forestry,Changsha,410004)
出处
《分子植物育种》
CAS
北大核心
2021年第6期1822-1829,共8页
Molecular Plant Breeding
基金
湖南省自然科学基金项目(2018JJ3280)
中国博士后科学基金面上资助(2018M632964)
长沙市科技计划项目(kq2004038)
长沙市木本花卉工程技术研究中心(kq1907081)共同资助。
关键词
灰毡毛忍冬
花蕾
花冠
转录组
Lonicera macranthoides
Flower bud
Corolla
Transcriptome
