摘要
随着测序技术的快速发展,整合DNA条形码和高通量测序的DNA宏条形码技术已经成为当前研究热点之一,在食草动物的食性鉴定中有很大潜力。放牧动物食性研究是动物营养学和草地生态学领域的重要研究内容。而与传统食性研究方法相比,宏条形码技术可通过对植物DNA条形码的高通量测序,获得样本中的物种组成进而分析动物食性。介绍了传统食性分析手段的局限,重点综述了DNA宏条形码技术的产生、操作原理以及在食草类动物食性鉴定领域中的应用,同时还简述了可能存在的挑战,并对该技术今后的发展方向进行了展望。
With the rapid development of sequencing techniques,DNA metabarcoding that derived from the combination of DNA barcoding and high-throughput sequencing technologies,has been a current research hotspot and shows great potential in diet identification of herbivores.Dietary analysis of grazing animal is a key research content in animal nutrition and grassland ecology.In contrast to traditional approach on diet determination,DNA metabarcoding can be used to investigate the species composition of a sample by high-throughput sequencing on plant DNA barcodes,and then to study the food diet of herbivores.This paper briefly reviewed the limitations of traditional diet analysis methods,and then summarized the background and analytical principles of DNA metabarcoding and its applications in the identification of herbivore diet.It also outlined the possible challenges in the research process and prospected the future trends of the DNA metabarcoding technology.
作者
郭艳萍
张浩
赵新钢
罗海玲
张英俊
GUO Yan-ping;ZHANG Hao;ZHAO Xin-gang;LUO Hai-ling;ZHANG Ying-jun(College of Animal Science and Technology,China Agricultural University,Beijing 100193;College of Grassland Science and Technology,China Agricultural University,Beijing 100193)
出处
《生物技术通报》
CAS
CSCD
北大核心
2021年第3期252-260,共9页
Biotechnology Bulletin
基金
宁夏回族自治区重点研发计划项目-现代人工草地高效利用关键技术研究与示范(2017BY082)
宁夏高端滩羊肉生产关键技术与集成示范(2018BBF02016)
国家肉羊产业技术体系(CARS-38)
中国博士后科学基金项目(2019M660877)。
关键词
植物条形码
宏条形码
高通量测序
食草动物
食性鉴定
plant barcode
metabarcoding
high-throughput sequencing
herbivore
diet identification
