摘要
目的评价丙泊酚对异相睡眠剥夺小鼠结肠能量代谢的影响。方法 SPF级健康雄性C57BL/6小鼠64只,6~8周龄,体重15~25 g。采用随机数字表法分为4组(n=16):对照组(C组)、睡眠剥夺组(SD组)、丙泊酚组(P组)和脂肪乳剂组(I组)。采用多平台水环境法制备小鼠异相睡眠剥夺模型,行异相睡眠剥夺72 h。P组和I组于异相睡眠剥夺模型制备完成后1 d分别腹腔注射丙泊酚150 mg/kg和等量10%脂肪乳剂,C组不处理。于模型制备后2 d时每组随机取小鼠,采用玻璃珠排出法评估结肠的运动功能。随后于12时(T1)和0时(T2)随机处死小鼠,取下丘脑视交叉上核(SCN)和结肠组织,采用Western blot法检测Per2和CLOCK表达,免疫荧光法检测结肠粘膜上皮细胞NAMPT表达。结果与C组比较,余3组玻璃珠结肠排出时间延长,T1时结肠NAMPT、SCN和结肠CLOCK、Per2表达上调,T2时CLOCK和Per2表达下调(P<0.05);与SD组比较,P组玻璃珠结肠排出时间缩短,T1时结肠NAMPT、SCN和结肠CLOCK表达上调,Per2表达下调,T2时CLOCK和Per2表达上调(P<0.05),I组差异无统计学意义(P>0.05);与P组比较,I组玻璃珠结肠排出时间延长,T1时结肠NAMPT、SCN和结肠CLOCK表达下调,Per2表达上调,T2时CLOCK表达上调,Per2表达下调(P<0.05)。结论丙泊酚可改善异相睡眠剥夺小鼠结肠能量代谢,其机制可能与改善结肠节律有关。
Objective To evaluate the effect of propofol on colonic energy metabolism in mice deprived of paradoxical sleep.Methods Sixty-four healthy male C57BL/6 mice,aged 6-8 weeks,weighing 15-25 g,were divided into 4 groups(n=16 each)using a random number table method:control group(group C),sleep deprivation group(group SD),propofol group(group P),and intralipid group(group I).The mice were deprived of paradoxical sleep for 72 h,which was induced by the multi-platform water environment method.In P and I groups,propofol 150 mg/kg and isovolemic 10%intralipid were intraperitoneally injected,respectively,for 2 h at 1 day after the model was established,and group C received no treatment.Mice were randomly selected from each group at 2 days after establishing the model,and the colonic motility of the mice was evaluated by timing the expulsion of glass beads from the colon.Then mice were randomly taken and sacrificed at 12 o′clock(T1)and 0 o′clock(T2).The hypothalamic suprachiasmatic nucleus(SCN)and colon tissues were removed,and the expression of Per2 and CLOCK was detected by Western blot,and the expression of NAMPT in colonic mucosa was detected by immunofluorescence.Results Compared with group C,the time of glass beads expulsed from the colon was significantly prolonged,the expression of NAMPT,SCN,CLOCK and Per2 was up-regulated at T1,and the expression of CLOCK and Per2 was down-regulated at T2 in the other three groups(P<0.05).Compared with group SD,the time of glass beads expulsed from the colon was significantly shortened,the expression of NAMPT,SCN and CLOCK was up-regulated at T1,the expression of Per2 was down-regulated at T1,and the expression of CLOCK and Per2 was up-regulated at T2 in group P(P<0.05),and no significant change was found in group I(P>0.05).Compared with group P,the time of glass beads expulsed from the colon was significantly prolonged,the expression of NAMPT,SCN and CLOCK was down-regulated at T1,the expression of Per2 was up-regulated at T1,and the expression of CLOCK was up-regulated and the
作者
常静
陈畅
陈婷
方鹏
王焱林
张宗泽
Chang Jing;Chen Chang;Chen Ting;Fang Peng;Wang Yanlin;Zhang Zongze(Department of Anesthesiology,Zhongnan Hospital,Wuhan University,Wuhan 430071,China)
出处
《中华麻醉学杂志》
CAS
CSCD
北大核心
2020年第11期1345-1348,共4页
Chinese Journal of Anesthesiology
基金
国家自然科学基金(81671060,81771160)。
关键词
二异丙酚
睡眠剥夺
能量代谢
结肠
Propofol
Sleep deprivation
Energy metabolism
Colon
