摘要
建立了新型整体柱固相微萃取-超高效液相色谱/串联质谱分析植物中10种痕量细胞分裂素的分析方法。通过1,4-二苯基丁二炔与六(3-巯基丙酸)二季戊四醇酯巯-炔点击聚合制备了通透性较好的固相微萃取整体柱,采用红外光谱、扫描电镜进行了表征。研究了最佳萃取条件和该方法对细胞分裂素的富集能力,结果表明,方法线性范围为0.0250~5.00μg/L,检出限在2.42~8.02 ng/L之间,相对标准偏差(RSD)小于6.2%。该方法应用于分析水稻叶和番茄根中细胞分裂素含量,加标回收率为82.2%~116.4%,RSDs为2.5%~7.9%。方法能满足植物中痕量细胞分裂素的分析检测。
A novel method was developed for the simultaneous analysis of 10 cytokinins in plants by monolithic column solid-phase microextraction(SPME) coupled with ultra-high performance liquid chromatography-tandem mass spectrometry.The polymer monolithic columns with better permeability was prepared by mercapto-acetylene click polymerization of 1,4-diphenylbutylene with dipentaerythritol hexakis(3-mercaptopropionate).The columns were characterized by infrared spectroscopy and scanning electron microscope.Extraction conditions and enrichment efficiency were investigated.The method showed a linear range of 0.0250-5.00 μg/L and a limit of detection of 2.42-8.02 ng/L,with the relative standard deviation(RSD) of less than 6.2%.The developed method was successfully applied for the determination of cytokinins in paddy leave and pomato root,with the recoveries of 82.2%-116.4% and the RSDs of 2.5%-7.9%,and can be used for trace determination of cytokinins in plant samples.
作者
王兴益
杨燕群
吴韵
郑玉国
张仟春
WANG Xingyi;YANG Yanqun;WU Yun;ZHENG Yuguo;ZHANG Qianchun(School of Biology and Chemistry,Xingyi Normal University for Nationalities,Xingyi 562400;Key Laboratory of Chemical Synthesis and Environmental Pollution Control-Remediation Technology of Guizhou Province,Xingyi Normal University for Nationalities,Xingyi 562400)
出处
《分析试验室》
CAS
CSCD
北大核心
2021年第3期275-280,共6页
Chinese Journal of Analysis Laboratory
基金
国家自然科学基金(21505115)
贵州省科学技术基金(黔科合LH字[2017]7026)
贵州省普通高等学校科技拔尖人才支持计划(黔教合KY字[2018]078)
贵州省教育质量提升项目(2017014)
贵州省教育厅青年科技人才成长项目(黔教合KY字[2017]365)
黔西南州科技局项目(2016-1-32)资助。
关键词
整体柱
固相微萃取
串联质谱
细胞分裂素
monolithic column
solid-phase microextraction
tandem mass spectrometry
cytokinins
