摘要
本研究采用Illumina HiSeq分子测序技术,探究了兴隆山东山丛枝菌根(Arbuscular mycorrhizal,AM)真菌多样性及其在不同海拔高度的分布特征。结果表明:1)自兴隆山东山土壤分离获得AM真菌共52种,隶属于4目8科13属,其中球囊霉属(Glomus)和原囊霉属(Archaeospora)为主要物种,分别为20种和9种,共占总物种的55.77%;2)各海拔梯度AM真菌种属分布结果表明,优势属球囊霉属(Glomus)和类球囊霉属(Paraglomus)随海拔变化呈现规律性分布,其余各属均匀分布于不同海拔梯度;球状巨孢囊霉(Gigaspora margarita)、蜜色无梗囊霉(Acaulospora mellea)、白色球囊霉(Glomus albidum)、球囊霉属的Glomus sp.1和Glomus sp.4,还有弯丝硬囊霉(Sclerocystis sinuosa)仅在部分海拔梯度出现,为不同海拔特有种;3)AM真菌丰富度分析表明,从低海拔到高海拔,ACE和Chao1指数总体上表现出“单峰”变化曲线,且部分海拔间差异性显著(P<0.05)。Spearman相关性分析发现,该地区AM真菌种属分布受海拔因子影响较小,两者间不具有显著相关性。综上,兴隆山东山AM真菌资源丰富,物种繁多,具有较大的应用潜力。
In this study,Illumina HiSeq molecular sequencing technology was used to examine the diversity of arbuscular mycorrhizal fungi and their distribution characteristics at different altitudes in the Xinglong Mountain Nature Reserve(East Mountain),Gansu Province.A total of 52 species of arbuscular mycorrhizal fungi belonging to four orders,eight families,and 13 genera were obtained from soil samples.Among these,species of Glomus and Archaeospora were predominant,being represented by 20 and 9 species,respectively,and accounting for 55.77%of the total species.Results obtained for the distribution of arbuscular mycorrhizal fungi at different altitudes revealed that the dominant genera Glomus and Paraglomus were regularly distributed with changes in altitude,whereas the remaining genera were uniformly distributed at different altitudes.Gigaspora margarita,Acaulospora mellea,Glomus albidum,Glomus sp.1,Glomus sp.4,and Sclerocystis sinuosa appeared only at certain elevation levels,and are assumed to be restricted to different elevations.Analysis of arbuscular mycorrhizal fungal species richness indicated that from low altitude to high altitudes,the ACE and Chao1 indices generally had a“single peak”change curve,and showed significant differences at certain altitudes(P<0.05).Spearman correlation analysis indicated that the distribution of arbuscular mycorrhizal fungi in this region is less affected by the altitude factor,and that there were no significant correlations between altitudes.In summary,arbuscular mycorrhizal fungi resources on the East Xinglong Mountain are rich and have considerable application potential.
作者
董万清
闫智臣
邓杰
李应德
丁婷婷
段廷玉
DONG Wanqing;YAN Zhichen;DENG Jie;LI Yingde;DING Tingting;DUAN Tingyu(State Key Laboratory of Grassland Agro-ecosystems/Key Laboratory of Grassland Livestock Industry Innovation,Ministry of Agriculture and Rural Affairs/College of Pastoral Agriculture Science and Technology,Lanzhou University,Lanzhou 730020,Gansu,China)
出处
《草业科学》
CAS
CSCD
北大核心
2021年第3期489-500,共12页
Pratacultural Science
基金
国家重点研发计划(2019YFC0507700)
国家绿肥产业技术体系(CARS-22)。
关键词
AMF
高通量测序
海拔
种属分布
资源调查
球囊霉属
arbuscular mycorrhizal fungus
high-throughput sequencing
altitude
species distribution
resources survey
Glomus
