摘要
目的了解2018-2019年宁夏皮肤炭疽疫情的流行特征及炭疽芽胞杆菌检出情况,为宁夏皮肤炭疽防控工作提供科学依据。方法使用描述性流行病学方法分析2018-2019年宁夏皮肤炭疽疫情的三间分布规律,采集疑似病例样本进行炭疽芽胞杆菌分离培养及Real-time PCR检测。结果2018-2019年共报告皮肤炭疽病例45例,时间集中在8月份(占57.78%),分布在11个县区(占50.00%),职业以农民为主(占58.00%)。对人体标本病原培养70份,共分离到炭疽芽胞杆菌12株,总检出率为17.14%;Real-time PCR检出率为64.29%,在患者使用抗生素前采样的标本分离率(64.29%)明显高于患者使用抗生素后采样的标本分离率(10.71%)。结论宁夏皮肤炭疽疫源地面积较广,处于较低散发水平,炭疽芽胞杆菌检出率较低,使用抗生素前采样检出率明显高于使用抗生素后,Real-time PCR可用于炭疽芽胞杆菌的辅助诊断。
This study aimed to understand the epidemiological characteristics of cutaneous anthrax and to determine the detection rate of Bacillus anthracis in Ningxia from 2018 to 2019,to provide a scientific basis for the prevention and control of cutaneous anthrax in Ningxia.Descriptive epidemiological methods were used to analyze the three distribution rule of cutaneous anthrax in Ningxia from 2018 to 2019.Samples from suspected cases were collected for isolation and culture of B.anthracis and real-time PCR detection.A total of 45 cases with cutaneous anthrax were reported from 2018 to 2019,which mainly occurred in August(57.78%),and were distributed in 11 counties(50.00%),in which peasants were the main occupational group(58%).A total of 12 B.anthracis strains were isolated from 70 human specimens,and the total detection rate was 17.14%.The detection rate with real-time PCR was 64.29%,and the isolation rate in samples collected before and after antibiotic administration was 64.29%,a proportion significantly higher than that in samples collected after antibiotic administration(10.71%).The area covered by cutaneous anthrax in Ningxia is relatively broad and has a low sporadic level.The detection rate of B.anthracis was low,and the positive detection rate in sampling before antibiotic administration was significantly higher than that after antibiotic administration.Real time PCR can be used for the auxiliary diagnosis of B.anthracis.
作者
杨聪
高建炜
马学平
韩坤
邹悦
张术斌
海娥
陈丽莉
YANG Cong;GAO Jian-wei;MA Xue-ping;HAN Kun;ZOU Yue;ZHANG Shu-bin;HAI E;CHEN Li-li(Ningxia Hui Autonomous Region Center for Disease Prevention and Control,Yinchuan 750004,China)
出处
《中国人兽共患病学报》
CAS
CSCD
北大核心
2021年第3期241-245,共5页
Chinese Journal of Zoonoses
基金
宁夏卫生健康委科研课题(No.52)。
关键词
炭疽
流行病学特征
实验室检测
anthrax
epidemiological characteristics
laboratory detection
