摘要
目的检测自发性高血压大鼠(SHR)并高尿酸血症降低尿酸干预血脂水平变化。方法动物实验分组:(1)对照组(A1)(单纯SHR组) n=20雌雄各半用等体积0.5%羧甲基纤维素钠(CMC-Na)灌胃两周。(2)SHR并高尿酸血症组(B1) n=30雌雄各半用腺嘌呤(150 mg/kg溶于0.5%CMC-Na)和乙胺丁醇(250 mg/kg溶于0.5%CMC-Na)灌胃两周;(3)继续造模对照组(A2)(单纯SHR组) n=10(从对照组A1随机选取),雌雄各半,在A1组干预完成后,再用等体积0.5%CMC-Na灌胃,继续灌胃5周。(4) SHR并高尿酸血症继续造模组(B2) n=10(从SHR并高尿酸血症组B1随机选取),雌雄各半,在B1组干预完成后,再用腺嘌呤(150 mg/kg溶于0.5%CMCNa)和乙胺丁醇(250 mg/kg溶于0.5%CMC-Na)继续灌胃5周。(5) SHR+高尿酸血症+尿酸降低组(B3) n=10(从SHR+高尿酸血症组B1随机选取),雌雄各半,在B1组干预完成后,再用腺嘌呤(150 mg/kg溶于0.5%CMC-Na)和乙胺丁醇(250 mg/kg溶于0.5%CMC-Na)和别嘌呤醇(10 mg/kg溶于0.5%CMC-Na)继续灌胃5周。在动物实验前,在动物实验2周,4周,7周。用酶标仪分别测定血中尿酸(UA)、胆固醇(TC)、甘油三酯(TG)、高密度脂蛋白胆固醇(HDL-C)、低密度脂蛋白胆固醇(LDL-C)、极低密度脂蛋白(VLDL)。结果实验组与对照组相比,血中尿酸水平、血脂水平有显著差异P<0.05。结论 SHR并高尿酸血症降低尿酸血脂水平有改变。
Objective To detect changes in serum lipid levels due to serum uric acid-lowering therapy in spontaneously hypertensive rats with hyperuricemia.Methods Experimental rats were divided into five groups.The control group(A1;spontaneously hypertensive rats;10 males,10 females)was administered the same volume of 0.5%carboxymethyl cellulose sodium for 2 weeks.The spontaneously hypertensive rats with hyperuricemia group(B1;15 males,15 females)was administered adenine(150 mg/kg dissolved in 0.5%carboxymethyl cellulose sodium)and ethambutol(250 mg/kg dissolved in 0.5%carboxymethyl cellulose sodium)for 2 weeks.The control/continuing established animal model group(A2;spontaneously hypertensive rats;5 males and 5 females randomly chosen from A1)received the same volume of 0.5%carboxymethyl cellulose for 4 weeks.The spontaneously hypertensive rats with hyperuricemia/continuing established animal model group(B2;5 males and 5 females randomly chosen from B1)were administered adenine(150 mg/kg dissolved in 0.5%carboxymethyl cellulose sodium)and ethambutol(250 mg/kg dissolved in 0.5%carboxymethyl cellulose sodium)for 5 weeks.The spontaneously hypertensive rats+hyperuricemia+serum uric acid-lowering therapy group(B3;5 males and 5 females randomly chosen from B1)were administered adenine(150 mg/kg dissolved in 0.5%carboxymethyl cellulose sodium)and ethambutol(250 mg/kg dissolved in 0.5%carboxymethyl cellulose sodium)and allopurinol(10 mg/kg Solved in 0.5%Carboxymethyl cellulose-Na)for 5 weeks.animal esperment in 2,4 and 7 weeks,before starting the experiment,the serum levels of uric acid,total cholesterol,triglycerides,high-density lipoprotein cholesterol,low-density lipoprotein cholesterol,and very-low-density lipoprotein cholesterol were tested using microplate readers.Results Serum uric acid levels and serum lipids in the experimental group differed significantly from those of the control group(P<0.05).Conclusions Serum uric acid-lowering therapy for spontaneously hypertensive rats with hyperuricemia altered the serum lipid leve
作者
田飞
张春
李江
TIAN Fei;ZHANG Chun;LI Jiang(the Department of Internal Medicine,Urumqi Friendship Hospital,Urumqi 830049,China;the Center of Animal Experiment,XinJiang Medical University,Urumqi 830011;Library,Xin Jiang Branch,the Chinese Academy Of Science,Urumqi 830011)
出处
《中国比较医学杂志》
CAS
北大核心
2021年第3期67-73,共7页
Chinese Journal of Comparative Medicine
基金
乌鲁木齐市科技计划项目(Y151310025)。
关键词
自发性高血压大鼠
血脂
高尿酸血症
降低尿酸
动物模型
spontaneously hypertensive rat
serum lipid
hyperuricemia
serum uric acid-lowering therapy
animal model
