摘要
目的初步探讨电化学方法检测循环游离DNA在甲状腺癌诊断中的作用以及其与TI-RADS分级、临床病理特征的相关性。方法选取114例甲状腺癌患者和35例甲状腺良性病变患者以及35例正常人血浆提取循环游离DNA,通过电化学方法检测血浆游离DNA(cfDNA)的电流吸附率水平(ir%),分析电流吸附率在甲状腺癌、良性病变患者以及正常人的差异,以及吸附率与各临床病理特征之间及甲状腺影响报告和数据系统分级(TI-RADS分级)的关系。结果甲状腺癌患者ir值较良性病变患者ir值更高(P <0.05),AUC=0.778。甲状腺癌患者血浆ir值比正常人ir值更高(P <0.05),AUC=0.723。甲状腺良性病变患者与正常人ir值差异无统计学意义(P=0.094)。灵敏度为61.4%,特异度为68.6%,准确度为64.1%,差异有统计学意义(P <0.05)。ir值<29组和ir值≥29组在癌灶大小、淋巴结转移、局部侵犯、多中心性中的差异无统计学意义(P> 0.05)。ir值≥29是甲状腺癌的独立危险因素(OR=1.244,P <0.01)。结论血浆cf DNA电流吸附率水平(ir值)对甲状腺癌的诊断具有一定的意义;ir≥29是甲状腺癌的一个独立危险因素;血浆cfDNA电流吸附率水平(ir值)与TI-RADS分级相关。
Objective To investigate the role of electrochemical detection of circulating free DNA in the diagnosis of thyroid cancer and its correlation with TI⁃RADS grading and clinicopathological features.Methods Circulating free DNA was extracted from plasma of 114 patients with thyroid cancer,35 patients with benign thyroid disease and 35 normal people.The current adsorption rate(ir%)of plasma free DNA(cfDNA)was detected by electrochemical method.The differences of current adsorption rate between patients with thyroid cancer,benign thyroid disease and normal people,as well as the relationship between adsorption rate and clinicopathological features and thyroid shadow were analyzed The relationship between response reporting and data system classifica⁃tion(TI⁃RADS classification).Results The ir value of thyroid cancer patients was higher than that of benign lesions patients(P<0.05),AUC=0.778.The IR value of patients with thyroid cancer was higher than that of patients with benign lesions(P<0.05),AUC=0.778.There was no significant difference in ir between patients with benign thyroid lesions and normal controls(P=0.094).The sensitivity,specificity and accuracy were 61.4%,68.6%and 64.1%,with statistically significant differences(P<0.05).There was no statistically significant difference in tumor size,lymph node metastasis,local invasion and multi⁃centrality between the ir value<29 group and the ir value≥29 group(P>0.05).ir value≥29 was an independent risk factor for thyroid cancer OR=1.244(P<0.01).Conclusion Plasma cfDNA current adsorption rate(ir value)has certain significance in the diagnosis of thyroid cancer.The index of ir≥29 is an independent risk factor of thyroid cancer.Plasma cfDNA current adsorption rate(ir value)sis correlated with TI⁃RADs grading.
作者
阚志文
黄子杰
崔亚云
钱立庭
KAN Zhiwen;HUANG Zijie;CUI Yayun;QIAN Liting(Department of Radiotherapy,Provincial Hospital Affiliated to Anhui Medical University,Hefei 230001,China;不详)
出处
《实用医学杂志》
CAS
北大核心
2021年第6期792-796,共5页
The Journal of Practical Medicine
基金
国家自然科学基金青年科学基金项目(编号:2018TJQN019)
中央高校基本科研业务费专项资金项目(编号:WK9110000071)
科技部重点研发项目(编号:2016YFB1000905)。
关键词
甲状腺癌
电化学方法
循环游离DNA
电流吸附率
诊断
thyroid cancer
electrochemical method
circulating free DNA
current adsorption rate
diagnosis
