摘要
目的应用网络药理学方法,探索皂角刺作用于乳腺癌的关键靶点及信号通路,为进一步研究提供基础和依据。方法通过检索数据库,收集皂角刺的化学成分及靶点、乳腺癌的作用靶点,并使用R语言,映射共同靶点。构建蛋白互作网络(PPI),提取关键靶点。使用R语言进行GO和KEGG富集分析。构建“成分-靶点”及“靶点-通路”网络,提取关键靶点及通路。结果筛选得到11个皂角刺活性成分,相关的靶点174个,乳腺癌相关靶基因1132个,共同靶点88个。富集分析得到GO条目104条,KEGG信号通路144条。PPI中的关键靶点有3个富集在关键通路中,靶点为:MAPK1、AKT1、RELA,涉及的通路包括:癌症、细胞凋亡、TNF信号通路、感染、IL-17信号通路和内分泌抵抗。结论皂角刺可能通过靶向相关通路上的MAPK1、AKT1、RELA发挥抗乳腺癌的作用。
Objective To explore the key targets and signal pathways of Gleditsiae Spina in the treatment of breast cancer and provide a basis and some evidence for further research.Methods Chemical components and targets of Gleditsiae Spina and the targets related to breast cancer were collected by searching databases.Common targets were mapped out by using R language.A protein interaction network(PPI)was constructed to extract key targets.GO and KEGG enrichment analysis were performed by using R language."Component-target"and"target-pathway"network was built to extract key targets and pathways.Results Eleven active ingredients and 174 targets of Gleditsiae Spina,1132 targets related to breast cancer and 88 common targets were obtained after screening.Total 104 GO entries and 144 KEGG signal paths were yielded by enrichment analysis.Three key targets in the PPI network were enriched in key pathways.The targets were MAPK1,AKT1,RELA and the pathways involved were cancer,cell apoptosis,TNF signaling pathway,infection,IL-17 signaling pathway and endocrine resistance.Conclusion Gleditsiae Spina may play an anti-breast cancer effect by targeting MAPK1,AKT1 and RELA in related pathways.
作者
权琦
陈平
黄圆圆
张蓓
QUAN Qi;CHEN Ping;HUANG Yuan-yuan;ZHANG Bei(Department of Traditional Chinese Medicine,Sun Yat-sen University Cancer Center,Guangdong Province,Guangzhou510060,China)
出处
《中国当代医药》
CAS
2021年第9期4-8,13,共6页
China Modern Medicine
基金
广东省医学科学技术研究基金项目(C2015049)。
关键词
皂角刺
网络药理学
乳腺癌
作用机制
Gleditsiae Spina
Network pharmacology
Breast cancer
Action mechanism
