摘要
GRAS转录因子在植物生长发育及抗逆境胁迫中具有重要作用。本文对大麻GRAS转录因子家族进行了全基因组鉴定,对其理化性质、系统进化发育、基因结构、基因间的共线性关系进行了分析,并利用云麻1号及内蒙古小粒大麻的转录组数据分析其在镉胁迫下的表达量变化。结果表明,大麻基因组中鉴定出54个GRAS基因,96.30%的基因编码酸性蛋白,长度为415~757 aa,分子质量为46,405.05~85,748.52 kD,等电点为4.77~8.54,划分为9个亚家族,其中PAT1、LS、SHR、HAM保守性较高,PAT1、LISCL、CsGRASA出现大量基因串联重复,CSGRAS12基因在5种植物的共线性分析中均存在。将2个大麻品种进行镉胁迫处理发现,云麻1号株高下降10.48%,鲜重下降6.33%;内蒙古小粒大麻株高下降66.07%,鲜重下降42.67%,表明云麻1号较内蒙古小粒大麻更耐镉胁迫。云麻1号的54个GRAS基因中,42个(77.78%)基因表达上调1.05~18.10,11个(20.37%)基因表达下调0.13~0.91;内蒙古小粒大麻的54个GRAS基因中,27个(50.00%)基因表达上调1.01~6.46,27个(50.00%)基因表达下调0.30~0.96。将两者GRAS基因进行同源基因鉴定并分析其表达情况发现,耐镉品种云麻1号中40个同源GRAS基因较内蒙古小粒大麻在镉胁迫下的上调或下调幅度更明显,表明这些GRAS基因与镉胁迫有明显相关性。本文可为挖掘和验证大麻GRAS基因提供参考。
GRAS transcription factors play important roles in plant growth,development and stress responses.In order to systematically analyze the GRAS transcription factor family in hemp,we performed a genome-wide identification of hemp GRAS genes,analyzed their physical and chemical properties,phylogenetic development,gene structure,and GRAS gene expression under cadmium stress in two hemp varieties(Yunma 1 and Inner Mongolia Xiaolidama).The results showed that there were 54 GRAS transcription factors in hemp genome,with the protein length from 415 to 757,encoding 96.30%of acidic proteins,the molecular weight from 46,405.05 to 85,748.52 kD and the isoelectric point from 4.77 to 8.54.These transcription factors were divided into nine subfamilies,among which,PAT1,LS,SHR,and HAM were more conserved,PAT1,LISCL,and CsGRASA had a large number of tandem repeats of genes,and CSGRAS12 genes were all present in collinearity analysis of five plants.Yunma 1 and Inner Mongolia Xiaolidama were treated with cadmium stress.Plant height and the fresh weight decreased in Yunma 1 and Inner Mongolia Xiaolidama,by 10.48%,6.33%and 66.07%,42.67%,respectively,indicating that Yunma 1 was more tolerant than Inner Mongolia Xiaolidama under cadmium stress.Among 54 GRAS genes in Yunma 1,42 genes(77.78%)were up-regulated by 1.05-18.10,and 11 genes(20.37%)were down-regulated by 0.13-0.91.Among 54 GRAS genes in small-grain cannabis in Inner Mongolia,27 genes(50.00%)were up-regulated by 1.01-6.46,and 27 genes(50.00%)were down-regulated by 0.30-0.96.This study showed that the 40 homologous GRAS gene in Yunma 1 were significanlty up-or down-regulated than those in Inner Mongolia Xiaolidama under cadmium stress,indicating that these GRAS genes were significantly related to cadmium stress.This study can provide a reference for subsequent mining and verification of GRAS genes in hemp.
作者
尹明
杨大为
唐慧娟
潘根
李德芳
赵立宁
黄思齐
YIN Ming;YANG Da-Wei;TANG Hui-Juan;PAN Gen;LI De-Fang;ZHAO Li-Ning;HUANG Si-Qi(Institute of Bast Fiber Crops,Chinese Academy of Agricultural Sciences/Key Laboratory of Biological and Processing for Bast Fiber Crops,Ministry of Agriculture and Rural Affairs,Changsha 410205,Hunan,China)
出处
《作物学报》
CAS
CSCD
北大核心
2021年第6期1054-1069,共16页
Acta Agronomica Sinica
基金
国家现代农业产业技术体系建设专项(CARS-16-E02)资助。
关键词
大麻
GRAS转录因子
系统发育分析
基因结构分析
表达分析
hemp
GRAS transcription factors
phylogenetic analysis
gene structure analysis
expression analysis
