摘要
天冬氨酸蛋白酶(aspartic proteinases,APs)是一类重要的蛋白水解酶,其在植物生长发育、抗病及逆境胁迫过程中发挥着重要的调控作用。目前,有关植物APs的研究报道较少,且在棉花中还没有相关报道。本研究基于陆地棉最新基因组数据库,共鉴定出87个APs(GhAPs)成员,并对其结构、启动子顺式作用元件及表达模式进行了分析。结果表明:陆地棉87个APs成员中,只有83个拥有两个完整的ASP保守位点;系统进化树分析将其聚类为典型(A)、类珠型(B)和非典型(C)三大类;染色体定位显示GhAPs基因在除A04外的所有染色体上均有分布,且发现5对串联重复基因;共线性分析发现,GhAPs基因间存在大量的共线性,且每个GhAPs基因与1~4个基因间存在共线性关系,这进一步说明GhAPs在进化过程中可能发生了基因复制事件,导致了APs家族的扩张。启动子顺式作用元件预测及组织表达分析结果表明,GhAPs基因可能参与调控陆地棉种子萌发、胚珠发育及花药形成等生长发育阶段,同时在抵御干旱、低温、伤口胁迫及响应ABA、GA、MeJA等激素调控过程中发挥重要作用。本研究结果为后期深入探究GhAPs功能,提高棉花产量、增强棉花耐逆性等方面研究提供一定的理论参考。
Aspartic protease(APs)is an important proteolytic enzyme,which plays an important regulatory role in plant growth and development,disease resistance and stress.However,functional mechanism of plant APs remains unclear,especially in cotton.In this study,87 APs(GhAPs)members were identified based on the latest upland cotton genome database,and their structure,promoter cis-acting elements and expression patterns were analyzed.Of the 87 APs,83 APs members in upland cotton had two complete ASP conserved loci,which were clustered into three categories:typical(A),nucellin-like(B)and atypical(C)by phylogenetic tree analysis.Chromosome mapping showed that GhAPs were distributed on all chromosomes except A04,and 5 pairs of tandem repeat genes were found.Collinear analysis showed that there were a large number of collinear among GhAPs genes,and there was a collinear relationship between each GhAPs gene and 1~4 genes,which further indicated that gene replication events may have occurred in the evolution of GhAPs,which led to the expansion of APs genefamily.The results of promoter cis-acting element prediction and tissue expression analysis showed that GhAPs may be involved in the regulation of upland cotton seed germination,ovule development and anther formation,and play an important role in resisting drought,low temperature,wound stress and responding to hormone regulation such as ABA,GA,MeJA and so on.The results of this study provide some theoretical reference for further exploring the function of GhAPs,improving cotton yield and enhancing cotton stress tolerance in the later stage.
作者
张亭亭
刘慧
黄先忠
Zhang Tingting;Liu Hui;Huang Xianzhong(Plant Genomics Laboratory,College of Life Sciences,Shihezi University,Shihezi,832003;College of Agriculture,Anhui Science and Technology University,Fengyang,233100;College of Life Sciences,Shihezi University,Shihezi,832003)
出处
《分子植物育种》
CAS
北大核心
2021年第5期1415-1429,共15页
Molecular Plant Breeding
基金
国家自然科学基金项目(31860393)
兵团中青年科技创新领军人才(2016BC001)共同资助。
