摘要
为创造转基因雄性不育种质,将缺失酶活性中心序列的红麻HcPDIL5-2a非全长基因通过花粉管通道法转化红麻保持系722B,获得了海南冬繁不育、南宁夏繁可育的低温敏雄性不育种质722THS,并从其姊妹交后代选育出了稳定型核不育(GMS)系722HS;此后,又以722HS作母本,以非转基因野生型722B为轮回亲本连续回交,选育出了细胞质雄性不育系722HA。对722THS与722HA的细胞学观察和线粒体DNA分子鉴定表明,二者的小孢子败育时期均为双核期,但722HA发生了线粒体DNA重排,而722THS与722B的线粒体DNA保持不变。本研究结果为转基因创造作物雄性不育种质找到了一条新途径,具有重要的科学意义和广阔的应用前景。
In order to create transgenic male sterile germplasm in kenaf,the partial length of gene HcPDIL-2a was transformed into maintainer line 722HB via pollen tube pathway.A low-temperature sensitive male sterile mutant called‘722THS’was accquired,which showed sterile when grown in winter in Hainan and fertile when grown in summer in Nanning,and a stable Genic Male Sterile(GMS)line 722HS was selected from its sister progenies.A cytoplasmic male sterile line 722HA was bred by crossing the mutant 722THS with the wild type 722B.Cytological observation indicated that the microspore abortion of 722THS and 722HA both happened at dinuclear stage.The mitochondrial DNA molecular tag revealed that mitochondrial DNA rearrangement occurred in 722HA,while mitochondrial DNA of 722THS and 722B remained unchanged.This study provided a new way for the creation of male sterile germplasms,which had important scientific significances and broad application prospects.
作者
周步进
李刚
金刚
周瑞阳
刘冬梅
汤丹峰
廖小芳
刘一丁
赵艳红
王颐宁
ZHOU Bu-Jin;LI Gang;JIN Gang;ZHOU Rui-Yang;LIU Dong-Mei;TANG Dan-Feng;LIAO Xiao-Fang;LIU Yi-Ding;ZHAO Yan-Hong;WANG Yi-Ning(College of Agriculture,Guangxi University,Nanning 530004,Guangxi,China;Maize Research Institute of Guangxi Academy of Agricultural Sciences,Nanning 530007,Guangxi,China;Guangxi Botanical Garden of Medicinal Plants,Nanning 530023,Guangxi,China;Cash Crop Institute of Guangxi Academy of Agricultural Sciences,Nanning 530007,Guangxi,China;Shangqiu Normal University,Shangqiu 476000,Henan,China)
出处
《作物学报》
CAS
CSCD
北大核心
2021年第6期1043-1053,共11页
Acta Agronomica Sinica
基金
国家自然科学基金项目(31571719)
广西自然科学基金项目(2018JJB130072)资助。
