摘要
目的:研究胰高血糖素样肽1(glucagon like peptide-1,GLP-1)能否通过影响CD36和酯酰辅酶A1(acyl-CoA1,ACAT1)的表达情况来减少巨噬细胞来源的泡沫细胞内胆固醇的含量。方法:从小鼠体内获取巨噬细胞,进行相应处理后分为4组:空白对照组、实验组、GLP-1激动组、GLP-1抑制组,并检测各组细胞内CD36、ACAT1 mRNA和蛋白的表达情况及总胆固醇(TC)、游离胆固醇(FC)及胆固醇酯(CE)的水平。结果:与空白对照组比较,实验组TC、FC、CE的含量及CD36、ACAT1 mRNA和蛋白表达明显提高(P<0.05);与实验组比较,GLP-1激动组TC、FC、CE的含量及CD36、ACAT1 mRNA和蛋白表达明显降低(P<0.05);与GLP-1激动组比较,GLP-1抑制组TC、FC、CE的含量及CD36、ACAT1 mRNA和蛋白表达明显升高(P<0.05)。结论:CD36和ACAT1的表达水平能够被GLP-1所抑制,并且在GPL-1的间接影响下,细胞内胆固醇的水平也会有所下降,使得巨噬细胞向泡沫细胞转化这一过程受到抑制,从而减缓动脉粥样硬化的发生发展。
Objective:To investigate whether glucagon like peptide-1(GLP-1)can reduce the cholesterol in foam cells derived from macrophages by affecting the expression of CD36 and acyl-CoA1(ACAT1).Methods:Macrophages were obtained from mice and divided into 4 groups after corresponding treatment including blank control group,experimental group,GLP-1 agitation group,GLP-1 inhibition group.Then the expression of CD36,ACAT1 mRNA and protein in cells of each group was detected.Expression and levels of total cholesterol(TC),free cholesterol(FC)and cholesterol ester(CE)were also detected.Re-sults:Compared with the blank control group,the content of TC,FC,CE and the expression of CD36,ACAT1 mRNA and protein in the experimental group were significantly increased(P<0.05);while compared with the experimental group,thecontent of TC,FC,CE,the expressions of CD36 and ACAT1 mRNA and protein in the GLP-1 excited group were significantly decreased(P<0.05).Compared with the GLP-1 agitation group,the contents of TC,FC and CE and the expression of CD36,ACAT1 mRNA and protein were significantly increased in the GLP-1 inhibition group(P<0.05).Conclusions:The expression levels of CD36 and ACAT1 can be suppressed by GLP-1.Under the indirect influence of GPL-1,the level of intracellular cholesterol will also be reduced,so that the process of macrophages to foam cells is suppressed and the development and progression of atherosclerosis are delayed.
作者
岳云飞
韩永魁
张奎
申晓彧
YUE Yun-fei;HAN Yong-kui;ZHANG Kui;SHEN Xiao-yu(Department of Cardiology,Shanxi Medical University Second Hospital,Taiyuan 030000,Shanxi Province,China)
出处
《海南医学院学报》
CAS
2021年第6期405-409,共5页
Journal of Hainan Medical University
基金
山西省重点研发计划项目(201903D421071)。
