摘要
[目的]为深入研究绒毛白蜡树响应白蜡窄吉丁危害的分子机制。[方法]以健康与受害的绒毛白蜡树树干韧皮部为试验材料,利用RNA-Seq测序技术对绒毛白蜡树韧皮部的转录组表达变化进行分析。[结果]通过对比健康与受害韧皮部的转录组数据,共鉴定出3388个DEGs,其中受害韧皮相对于健康白蜡树韧皮表达上调的DEGs有2141个,表达下调的DEGs为1247个;通过GO功能注释,将差异基因划分为20个功能类别,包括细胞过程、代谢过程、催化活性、结合元件、转运活性以及核酸结合转录因子活性等;对差异表达基因分别进行KEGG代谢途径及功能富集分析,其中代谢途径共20个,包括碳水化合物代谢,氨基酸代谢,能量代谢和脂质代谢等,另外差异表达基因分别在122条通路中均有富集,包括植物-病原体互作、代谢进程、碳水化合物结合、核酸结合转录因子活性等;通过转录组家族统计,发现健康与受害白蜡树韧皮部共有20个转录因子家族,其中C3H、BHLH、NAC、MYB、B3、GRAS和SBP等转录因子家族基因表达量均达到显著差异。[结论]研究结果为揭示白蜡树应对虫害胁迫反应的分子机制提供分子与理论依据。
[Objective]To study the molecular response mechanism of Fraxinus velutina Torr to the infection of Agrilus planipennis Fairmaire.[Method]The phloem of non-infested and infested F.velutina stem were used as the experimental materials to analyze the transcriptome expression changes of the phloem using RNA-Seq sequencing technology.[Result]By comparing the transcriptome data of non-infested and infested phloem,a total of 3388 DEGs were identified,among which 2141 were up-regulated and 1247 were down regulated.Through GO functional annotation,the differential genes were divided into 20 functional categories,including cellular processes,metabolic processes,catalytic activities,binding elements,transport activities and nucleic acid binding transcription factor activities.The metabolic pathways and functional enrichment of KEGG were analyzed.There were 20 metabolic pathways,including carbohydrate metabolism,amino acid metabolism,energy metabolism and lipid metabolism.In addition,differentially expressed genes were enriched in 122 pathways,including plant-pathogen interactions,metabolic processes,carbohydrate binding,nucleic acid-binding transcription factor activities.According to transcriptome family statistics,it was found that there were 20 transcription factor families in phloem of non-infested and infested F.velutina,among which C3H,bHLH,NAC,MYB,B3,GRAS and SBP were significantly different.[Conclusion]The study will provide theoretical basis for revealing the molecular response mechanism of F.velutina to insect stress.
作者
封润霞
赵婕
张苏芳
王建军
魏建荣
刘建凤
FENG Run-xia;ZHAO Jie;ZHANG Su-fang;WANG Jian-jun;WEI Jian-rong;LIU Jian-feng(College of Life Sciences,Hebei University,Baoding 071000,Hebei,China;Research Institute of Forest Ecology,Environment and Protection,Chinese Academy of Forestry,Beijing 100091,China;Liaoning Academy of Forestry Sciences,Shenyang 110032,Liaoning,China)
出处
《林业科学研究》
CSCD
北大核心
2021年第1期47-55,共9页
Forest Research
基金
国家重点研发计划资助(2018YFC1200400)。
关键词
白蜡树
白蜡窄吉丁
韧皮部
转录组
Fraxinus velutina
Agrilus planipennis
phloem
transcriptome
