摘要
莺歌海盐场是中国华南地区重要的食用盐生产基地。本研究从莺歌海盐场土壤中分离到可培养细菌及真菌,采用16S rDNA、ITS序列构建系统发育树,分析菌株分类学地位。结果表明,10株细菌菌株分为3个类群,菌株YGHYCB1、YGHYCB2、YGHYCB4、YGHYCB6、YGHYCB9与Bacillus acidicola、B.shackletonii及B.pseudomycoides亲缘关系较近;菌株YGHYCB1与B.amyloliquefaciens、B.velezensis聚类;菌株YGHYCB3、YGHYCB7、YGHYCB8、YGHYCB10与BreviBacillus limnophilus聚类。12株真菌菌株分为4个类群,菌株YGHYCF1与Phragmotaenium spp.聚类;菌株YGHYCF2、YGHYCF4、YGHYCF7、YGHYCF12与Sporobolomyces spp.聚类;菌株YGHYCF5、YGHYCF6、YGHYCF9、YGHYCF10与Dermatosorus cyperi聚类;菌株YGHYCF3、YGHYCF8、YGHYCF11与Moesziomyces、Pseudozyma、Oryza、Macalpinomyces 4个不同属聚为一大支,展现了这3株菌株在分类学地位上的差异。以上结果反映了莺歌海盐场土壤中可培养真菌资源多样性丰富,而细菌由于生长受到高盐环境抑制,多样性不显著。
Yinggehai Saltern is an important salt production base in South China.In this study,culturable bacteria and fungi were isolated from the soil of Yinggehai saltern.Phylogenetic tree was constructed by 16 S rDNA and ITS sequences,and the taxonomy of the isolates were analyzed.The results show that 10 bacterial isolates were divided into 3 groups.The isolates YGHYCB1,YGHYCB2,YGHYCB4,YGHYCB6 and YGHYCB9 were closely related to Bacillus cacidicola,B.shackletouii and B.pseudomycoides;YGEHYCB1 clustered with B.amyloliquefacieus and B.velezensis;YGHYCB3,YGHYCB7,YGHYCB8,YGHYCB10 clustered with BreviBacillus limuophilus.Twelve fungal isolates were divided into 4 groups.YGHYCF1 clustered with Phragmotaeuium spp.;YGHYCF2,YGHYCF4,YGHYCF7,YGHYCF12 clustered with Sporobolomyces spp.;YGHYCF5,YGHYCF6,YGHYCF9,YGHYCF10 clustered with Dermatosorus cyperi;YGHYCF3,YGHYCF8,YGHYCF11 and Moesziomyces,Pseudozyma,Oryza,Macalpinomyces were clustered into one clade,which showed the difference of taxonomy among 3 isolates.The above results indicated the culturable fungi resources in the soil of Yinggehai Saltern are quite diverse,while the bacteria growth is inhibited by the high-salt environment,and the diversity is not significant.
作者
陈永敢
符明
林佳
李珍
罗宏伟
黄海
Chen Yonggan;Fu Ming;Lin Jia;Li Zhen;Luo Hongwei;Huang Hai(College of Fisheries and Life Science,Hainan Tropical Ocean University,Sanya,572022;Key laboratory of Utilization and Conservation for Tropical Marine Bioresources,Ministry of Education,Hainan Tropical Ocean University,Sanya,572022;Hainan Key Laboratory for Conservation and Utilization of Tropical Marine Fishery Resources,Hainan Tropical Ocean University,Sanya,572022)
出处
《基因组学与应用生物学》
CAS
CSCD
北大核心
2020年第12期5593-5598,共6页
Genomics and Applied Biology
基金
海南省重点研发计划项目(ZDYF2019212)
海南省自然科学基金项目(318MS072)
海南热带海洋学院科研项目(RHDQN201827)共同资助。
关键词
盐场
可培养微生物
多样性
Saltern
Culturable microorganisms
Diversity
