摘要
【目的】研究新疆阿克苏地区盐碱土样中细菌类群多样性和优势种群,及其与环境因子的相关性。【方法】采用基于16S rDNA的变性梯度凝胶电泳(Denaturing gradient gel electrophoresis,DGGE)、克隆测序和典型相关性分析(CCA)的方法。【结果】优势菌群序列分析表明有29个序列属于未培养的微生物,其他43个序列分属细菌的9个目:粘球菌目(Myxococcales)、假单胞菌目(Pseudomonadales)、根瘤菌目(Rhizobiales)、芽孢杆菌目(Bacillales)、伯克氏菌目(Burkholderiales)、放线菌目(Actinomycetales)、海洋螺菌目(Oceanospirillales)、黄杆菌目(Flavobacteriales)、交替单胞菌目(Alteromonadales),21个属。【结论】新疆阿克苏地区土壤中的微生物丰富度非常高,存在大量的细菌类群,优势菌群不尽相同,盐碱土样中微生物群落结构与环境因子是密切相关的。
[Objective] The research was conducted to study on the bacterial community di-versity and dominant population in Xinjiang akesu area and the correlation with the environ-mental factor.[Methods] The bacteria diversity and dominant population was analysis by PCR-denaturing gradient gel electrophoresis(DGGE),cloning,sequencing and the DGGE se-quence analysis and the canonical correlation analysis(CCA).[Results] The sequencing of the dominant population showed that there were 29 sequencing belong to the un-cultivation mi-crobial,and the other 43 DGGE band were made up by 9 order: Myxococcales,Pseudomo-nadales,Rhizobiales,Bacillales,Burkholderiales,Actinomycetales,Oceanospirillales,Fla-vobacteriale,Alteromonadales.[Conclusion] Canonical correlation analysis(CCA) result showed that microbial community structure and environmental factors were closely related in saline-alkaline soils.
出处
《微生物学通报》
CAS
CSCD
北大核心
2012年第7期1031-1043,共13页
Microbiology China
基金
国家自然科学基金项目(No.31170009)
广东省自然科学基金项目(No.10151007002000008)
新疆生产建设兵团塔里木盆地生物资源保护利用重点实验室开放基金课题重点项目(No.BRZD1004)
