摘要
目的:研究白花蛇舌草对病毒肺炎小鼠免疫细胞因子表达的影响。方法:选取48只昆明种小鼠,分为正常组、模型组、白花蛇舌草组、病毒唑组,每组12只。模型组、白花蛇舌草组和病毒唑组均以25μL(流感病毒新鲜尿囊液10倍系列稀释成1×10-4)流感病毒滴鼻感染小鼠,构建肺炎小鼠模型。构模后,白花蛇舌草组以3.0825 g/kg白花蛇舌草灌胃;病毒唑组以10 mg/kg病毒唑灌胃,均每天灌胃1次,连续7 d。正常组小鼠以等量的生理盐水灌胃处理。7 d后,称取体质量和肺组织质量,计算肺指数。收集血液和肺组织,用酶联免疫吸附法(ELISA)测定TNF-α、IL-6含量。收集肺泡灌洗液,计数白细胞数及分类。用Western blot方法测定肺组织中Toll样受体4(TLR4)、核因子-κBp65(NF-κB p65)、细胞间粘附分子-1(ICAM-1)、血管细胞粘附分子-1(VCAM-1)蛋白水平。结果:与正常组比较,模型组小鼠肺指数升高,血和肺组织中TNF-α、IL-6含量升高,肺泡灌洗液中白细胞总数、单个核细胞数、中性粒细胞数目均增多,肺组织中TLR4、NF-κB p65、ICAM-1、VCAM-1蛋白表达水平升高(均P<0.05)。与模型组比较,白花蛇舌草组和病毒唑组小鼠肺指数降低,血清和肺组织中TNF-α、IL-6含量降低,肺泡灌洗液中白细胞总数、单个核细胞数、中性粒细胞数目均减少,肺组织中TLR4、NF-κB p65、ICAM-1、VCAM-1蛋白表达水平降低(均P<0.05)。结论:白花蛇舌草可以降低肺炎小鼠免疫细胞因子表达,其机制可能与TLR4/NF-κB信号轴和粘附分子表达有关。
Objective:To study the effect of Hedyotis diffusa willd on the expression of immune cytokines in mice with pneumonia.Methods:A mouse model of pneumonia was established by infecting mice with influenza virus nose drop infection of 25μL(diluted to 10-4),the stomach was treated with Hedyotis diffusa Willd(3.0825 g/kg,200μL,gavage)once a day for 7 days.The body weight and lung tissue mass of mice were weighed,the lung index calculated,and serum and lung tissue were collected.The contents of TNF-αand IL-6 were determined by ELISA.Bronchoalveolar lavage fluid was collected,and leukocytes were counted and classified.The levels of TLR4,NF-κB p65,ICAM-1 and VCAM-1 proteins in lung tissues were measured by Western blotting.Results:As compared with normal mice,the lung index of mice with influenza virus pneumonia,the levels of TNF-αand IL-6 in serum and lung tissues,the total number of leukocytes,the number of mononuclear cells and the number of neutrophils in the bronchoalveolar lavage fluid,and the expression levels of TLR4,NF-κB p65,ICAM-1 and VCAM-1 protein in lung tissue increased.Treatment with Hedyotis diffusa willd could reduce the lung index of pneumonia mice,the levels of TNF-αand IL-6 in serum and lung tissue,the total number of leukocytes,the number of mononuclear cells and the number of neutrophils in bronchoalveolar lavage fluid,and the expression levels of TLR4,NF-κB p65,ICAM-1 and VCAM-1 in lung tissue.Conclusion:Hedyotis diffusa willd can reduce the expression of immune cytokines in pneumonia mice,which may be related to TLR4/NF-κB signal axis and adhesion molecules.
作者
马丽亚
董晶晶
高智星
张晓谊
MA Li-ya;DONG Jing-jing;GAO Zhi-xing;ZHANG Xiao-yi(Urumqi Traditional Chinese Medicine Hospital,Urumqi 830000,China)
出处
《内科急危重症杂志》
2021年第1期50-53,共4页
Journal of Critical Care In Internal Medicine
基金
乌鲁木齐市沙依巴克区科学基金资助项目(No:20130112)。
关键词
肺炎
白花蛇舌草
炎症
细胞因子
Pneumonia
Hedyotis diffusa Willd
Inflammation
Cytokines
