摘要
目的通过对白花蛇舌草及其2种地方习用品(伞房花耳草、纤花耳草)的性状、显微特征和HPLC指纹图谱全面分析比较,对三者进行鉴别研究。方法采用传统方法观察三者性状,鉴别茎横切面、叶横切面、果实横切面、种子横切面及药材粉末特征;采用HPLC指纹图谱比较三者色谱峰差异。结果性状项下,白花蛇舌草茎为圆柱形,蒴果单生或双生于叶腋,扁球形,直径2~3 mm,果柄长;伞房花耳草和纤花耳草为四棱柱形,伞房花耳草为2~5蒴果呈伞房花序生于叶腋,球形,直径1~1.5 mm,具细长的柄;纤花耳草1~3蒴果簇生于叶腋,卵圆形,边缘有纵棱,直径约1.5 mm,无柄,叶干时边缘反卷呈长针状。鉴别项下,白花蛇舌草茎横切面呈类圆形,叶中脉处下方突起,内果皮纤维层由2层纤维细胞组,种皮细胞表面观为多边形,壁上密布细小红棕色或黄棕色的疣状点;伞房花耳草茎横切面为四棱形,种皮细胞表面观为多边形,壁波状弯曲,壁上没有疣状点;纤花耳草中脉上方略四陷,下方不突起;内果皮纤维层由8~13层纤维细胞组成。HPLC指纹图谱分析结果显示,白花蛇舌草与常见地方习用品有各自特征色谱峰,有一定差别。结论传统和现代方法均显示白花蛇舌草及伞房花耳草、纤花耳草在性状、组织显微横切面、粉末特征、化学成分方面存在一定差异,可有效区分白花蛇舌草及其2种地方习用品。
Objective To distinguish Hedyotis diffusa Willd.and its common countrerfeit,Hedyotis corymbosa.and Hedyotis tenelliflora.by analyzing and comparing their macroscopical identification,microscopic character and HPLC fingerprints.Methods The features of macroscopical identification,microscopic character including cross-sections of stem,leaf,fruit and seed,and herbal powders were observed in the three samples by traditional methods.The difference of chromatographic peaks among the three samples were also analyzed by HPLC methods.Results The stems of Hedyotis diffusa Willd.were cylindrical,and the capsules were solitary or double born in the leaf axils,oblate,2-3 mm in diameter,with a long petiole;the Hedyotis corymbosa.and Hedyotis tenelliflora.were tetragonal,and the Hedyotis corymbosa.was 2-5 capsules born in leaf axils in corymbose inflorescences,globular,1-1.5 mm in diameter,with a slender petiole;the Hedyotis tenelliflora.were 1-3 capsules clustered in the leaf axils,ovoid with longitudinal ribs around the margin,about 1.5 mm in diameter,without the long petiole,about 1.5 mm in diameter,sessile,the edge of the leaf drying revolute long needle-like.Under the identification,the cross section of the Hedyotis diffusa Willd.stem was almost round,the middle vein of the leaves was protrusion below,the inner pericarp fiber layer consisted of two layers of fiber cells,the surface of the seed coat cells was polygon,and the wall was densely covered with small reddish brown or yellow-brown warty spots.The cross section of the Hedyotis corymbosa.stem was quadrilateral,the surface of the seed coat cell was polygon,the wall was wavy and curved,and there was no warty point on the wall.The middle veins of the Hedyotis tenelliflora.were slightly sunken in the upper part,but not protruding in the lower part;the endocarp fiber layer consisted of 8 to 13 layers of fiber cells.Moreover,the HPLC fingerprint analysis demonstrated substantial dissimilarities in the characteristic peaks of these herbs.Conclusion The traditional and moder
作者
辛雪颖
范崇庆
金艳
冯学锋
李娆娆
李海霞
Xin Xueying;Fan Chongqing;Jin Yan;Feng Xuefeng;Li Raorao;Li Haixia(Biopharmaceutical Center,Institute of Chinese Materia Medica,China Academy of Chinese Medical Sciences,Beijing 100700,China;College of Pharmacy,Zhengzhou University,Zhengzhou 450001,China;ShangHai Zenith Data Tech Co.,Ltd.,Shanghai 201100,China;National Resource Center for Chinese Materia Media,China Academy of Chinese Medical Sciences,Beijing 100700,China)
出处
《国际中医中药杂志》
2024年第4期498-505,共8页
International Journal of Traditional Chinese Medicine
基金
北京市自然科学基金(7112097)
中国中医科学院中药研究所“使命导向改革”重点专项(CI2023E001TS03-2-3)。
关键词
中药性状鉴定
中药显微鉴定
白花蛇舌草
伞房花耳草
纤花耳草
HPLC指纹图谱鉴别
Traditional Chinese drug characteristic identification
Traditional Chinese drug microscopic identification
Hedyotis diffusa Willd.
Hedyotis corymbosa(L.)Lam
Hedyotis tenelliflora Blume
HPLC fingerprints
