摘要
The flower bud of Tussilago farfara L.,also known as Farfarae Flos(FF),is commonly used in the traditional Chinese medicine(TCM)for the treatment of cough,bronchitis and asthmatic disorders.In this study,26 samples from five provinces across northern China were collected,and internal transcribed spacer-polymerase chain reaction(ITS-PCR)coupled with high performance liquid chromatography(HPLC)fingerprint profiling was used for the quality evaluation of FF.The results of the ITS sequence analysis showed the high similarity values among these samples.ITS2 sequence exhibited less molecular diversity compared with ITS1,and no obvious correlation was found between the variation of ITS with the production areas or cultivation methods.The results of HPLC fingerprints in combination with the similarity analysis suggested that relative contents of phenylpropanoids and flavonoids varied among the 26 FF samples,and there was also no obvious difference among the different habitats.The phenylpropanoids and the flavonoids showed similar fluctuating patterns,and positive correlation among them was also observed.The results presented in this study suggested that FF from different habits showed high similarities,and ITS-PCR coupled with HPLC fingerprint profiling could be used as a valuable approach for the quality evaluation of FF.
款冬花为菊科植物款冬(Tussilago farfara L.)的干燥花蕾,临床用于治疗咳嗽、支气管炎和哮喘。本研究收集了来自中国北方五个省的26批款冬花样本,并采用ITS-PCR与高效液相色谱(HPLC)指纹图谱相结合用于款冬花的质量评价。ITS序列分析结果表明这些样品之间具有很高的相似度。与ITS1相比,ITS2序列的分子多样性较低,并且ITS的变异与产地或栽培方法之间无明显的相关性。HPLC指纹图谱与相似度分析结果展示了26批款冬花样品中苯丙素类化合物和黄酮类化合物的相对含量变化趋势,并且这两类成分在不同产地之间无明显差异。苯丙素类化合物和黄酮类化合物显示出相似的变化趋势,且两者之间存在正相关。本研究表明,来自不同产地的款冬花呈现较高的相似度,且ITS-PCR结合HPLC指纹图谱分析的方法可以作为款冬花质量评价的一种有效方法。
基金
National Natural Science Foundation China(Grant No.31270008 and 81973466)
National Standardization Program of Traditional Chinese Medicine(Grant No.ZYBZH-Y-JIN-34)。
