摘要
目的:观察补肾活血法对系统性红斑狼疮(SLE)合并动脉粥样硬化模型小鼠免疫功能和主动脉斑块的影响。方法:8周龄大雌性载脂蛋白E基因敲除小鼠(ApoE^(-/-)mice)45只,给予一次性腹腔注射降植烷(pristane)0.5ml,继续喂养16周后,小鼠随机分为中药治疗组、西药治疗组和模型组3组各15只,以同周龄C57BL/6小鼠作为正常对照组。结果:与正常对照组比较,模型组尿素氮、肌酐、胆固醇和甘油三酯、免疫球蛋白IgG水平明显升高,补体C3水平下降,脾脏质量和蛋白尿增加,主动脉有较大斑块形成;中药治疗组和西药治疗组均能改善肾功能、蛋白尿、脾肿大,2组比较差异无统计学意义;中药治疗组在降低胆固醇和甘油三酯水平、IgG水平方面效果优于西药治疗组;中药治疗组与西药治疗组更能显著改善主动脉粥样硬化斑块病变范围。结论:与传统西药比较,补肾活血法对SLE合并动脉粥样硬化模型鼠的降血脂作用,改善免疫球蛋白水平和动脉斑块方面作用更明显。
Objective:To observe the effect of kidney-tonifying and blood-activating method on immune function and aortic plaque in mice with systemic lupus erythematosus and atherosclerosis.Methods:Forty-five eight-week-old female apolipoprotein E-gene knockout mice were intraperitoneally injected with 0.5ml norphytane.After 16 weeks of feeding,the mice were randomly divided into three groups,the Chinese medicine treatment group,the Western medicine treatment group and the Model group,15 mice in each group.C57BL/6 mice of the same age were used as normal Control group.Results:Compared with the Control group,the levels of urea nitrogen,creatinine,cholesterol and triglyceride,immunoglobulin IgG in the model group were significantly increased.The level of complement C3 was decreased,the spleen weight and proteinuria were increased,and the aorta had larger plaque formation.Both the Chinese medicine treatment group and the Western medicine treatment group could improve renal function,albuminuria and splenomegaly,and there was no significant difference between the two groups.The Chinese medicine treatment group was better than the Western medicine treatment group in lowering cholesterol,triglyceride levels and IgG levels.Compared with the Western medicine treatment group,the Chinese medicine treatment group could significantly improve the scope of aortic atherosclerotic plaques.Conclusions:Compared with the Western medicine therapy,kidney-tonifying and blood-activating therapy has more obvious effects on lowering blood lipid,improving the level of immunoglobulin and arterial plaque in SLE with atherosclerosis model mice.
作者
杨月
田园
陈玥颖
李永明
钟沈煜
薛鸾
YANG Yue;TIAN Yuan;CHEN Yue-Ying;LI Yong-ming;ZHONG Shen-yu;XUE Luan(Department of rheumatism,Yueyang hospital of integrated traditional Chinese and western medicine affiliated to Shanghai university of traditional Chinese medicine,Shanghai 200437,China)
出处
《中国中医基础医学杂志》
CAS
CSCD
北大核心
2021年第2期240-243,共4页
JOURNAL OF BASIC CHINESE MEDICINE
基金
国家自然科学基金青年基金项目(81704032)-从程序性死亡分子信号通路研究补肾活血法对系统性红斑狼疮早发动脉粥样硬化的抗炎及免疫调节作用
上海市卫生和计划生育委员会科研课题面上项目(201640238)-从程序性死亡分子信号通路研究补肾活血法对系统性红斑狼疮早发动脉粥样硬化的抗炎及免疫调节作用
国家自然科学基金面上项目(81573925)-基于PD-1/PD-L1通路对Treg细胞的调控探讨解毒通络生津方治疗干燥综合征的作用机制。
关键词
补肾活血法
系统性红斑狼疮
动脉粥样硬化
Kidney nourishing and blood activating therapy
Systemic lupus erythematosus
Atherosclerosis
