摘要
目的挖掘中医药治疗系统性红斑狼疮(SLE)的处方用药规律,并探索核心配伍药物可能的作用机制。方法检索中国知网(CNKI)、万方数据(WanFang Data)知识服务平台、维普(VIP)中文科技期刊数据库、中国生物医学(CBM)文献数据库中中医药治疗SLE的文献,构建方药数据库,通过频次统计、关联规则、系统聚类及因子分析对处方用药规律进行分析,通过BATMAN-TCM对核心配伍药物进行疾病富集及KEGG通路分析。结果329首处方涉及中药317味,涉及19大类,清热药、补虚药、活血化瘀药位居前3位,用药频次达4687次,频数>40次共计30味。关联规则得到二项关联组合27项,三项关联组合21项,关联网络图显示生地黄—牡丹皮之间关系最密切。系统聚类得到18组药物聚类组合。因子分析共提取出21个公因子。生地黄—牡丹皮配伍富集的生物学通路有47条,与SLE相关的信号通路有3条。结论SLE以阴虚内热为本,毒、热、瘀为标,治法以养阴清热为核心,核心药物配伍的作用机制可能是通过调节腺苷酸活化蛋白激酶(AMPK)、酶磷脂酰肌醇3-激酶/蛋白激酶B(PI3K/Akt)和神经活性配体受体相互作用信号通路实现。
Objective To observe the prescription medication laws of Chinese medicine in the treatment of systemic lupus erythematosus(SLE),and to study possible mechanism of action of core compatible herbs.Methods Retrieved were the literature on the treatment of SLE with Chinese medicine in China National Knowledge Infrastructure(CNKI),WanFang Data Knowledge Service Platform(WanFang D a ta),VIP Chinese Scientific Journal Database(VIP),China Biology Medicine Literature Database(CBM).And a prescription database was built up.Prescription medication laws were analyzed by frequency statistics,association rules,systemic clustering,and factor analysis.Disease enrichment and KEGG pathway analysis on core compatible herbs were performed using BATMAN-TCM.Results The 329 prescriptions covered 317 flavors of Chinese herbs,involving 19 categories.Heat-clearing herbs,tonic herbs,and blood stasis promoting herbs ranked the top 3.The frequency of medication was 4687,and totally 30 flavors occurred with the frequency over 40.According to the association rules,27 two-item association combinations and 21 three-item association combinations were obtained.The association network diagram showed that the relationship between Rehmannia glutinosa and Moutan cortex was the closest.Systemic clustering obtained 18 drug cluster combinations.A total of 21 common factors were extracted by factor analysis.There were 47 biological pathways enriched by the compatibility of Rehmannia glutinosa and Moutan cortex.There were 4 signal pathways related to SLE.Conclusions Yin deficiency and internal heat was the essence of SLE,toxin,heat,and blood stasis were its superficialities.Nourishing yin and clearing away heat could be taken as the core of the treatment methods.The mechanism of core drugs compatibility might be achieved by regulating the signal pathways of adenylate-activated protein kinase(AM PK),enzyme phosphatidylinositol 3-kinase/protein kinase B(PI3K/Akt),and neuroactive ligand receptor interactions.
作者
郭锦晨
刘健
付书璠
冯烨
GUO Jin-chen;LIU Jian;FU Shu-fan;FENG Ye(Graduate School,Anhui University of Traditional Chinese Medicine,Hefei,230012;Key Laboratory,Xin'an Medical Ministry of Education,Hefei,230038;Department of Rheumatology,First Affiliated Hospital,Anhui University of Traditional Chinese Medicine,Hefei,230031;Department of Hematology,First Affiliated Hospital,Guangzhou University of Traditional Chinese Medicine,Guangzhou,510405)
出处
《中国中西医结合杂志》
CAS
CSCD
北大核心
2020年第9期1052-1058,共7页
Chinese Journal of Integrated Traditional and Western Medicine
基金
国家中医药重点学科中医痹病学建设项目(No.国中医药发[2009]30号)
国家临床重点专科中医风湿病科建设项目(No.财社[2013]239号)。
关键词
系统性红斑狼疮
中药
数据挖掘
网络药理学
用药规律
机制
systemic lupus erythematosus
Chinese herbs
data mining
network pharmacology
medication laws
mechanism
