摘要
目的观察肢体缺血—再灌注(LIR)损伤过程中腹腔注射氢气的兔血清肿瘤坏死因子-相关凋亡诱导配体(TRAIL)、线粒体丝氨酸蛋白酶(Omi/HtrA2)及骨骼肌组织匀浆上清Omi/HtrA2的水平变化,并探讨其可能作用机制。方法 45只新西兰大耳白兔随机分为LIR+氢气组(H组)、LIR组(I组)及对照组(C组),每组各15只。H、I组将袖带绑缚于兔右后肢根部股动脉搏动处(超过收缩压20~30 mmHg可完全阻断血流),束缚4 h时打开球囊开关(再灌注),H组于再灌注前5 min腹腔注射5 mL/kg氢气,C、I组同时刻腹腔注射等体积空气。C组仅用相同袖带缠缚相同部位。分别于再灌注24、72、168 h时取各组大耳白兔,分别取静脉血及胫骨前肌肌组织,检测血清TRAIL、Omi/HtrA2及骨骼肌组织Omi/HtrA2。结果与I组比较,再灌注24、72、168 h时H组兔血清TRAIL、Omi/HtrA2水平低(P均<0.01);与I组比较,再灌注24、72、168 h时H组兔骨骼肌组织匀浆上清Omi/HtrA2水平降低(P均<0.01)。同组不同时间段I、H组血清TRAIL及其骨骼肌组织匀浆上清Omi/HtrA2间比较,P均<0.01,I组血清Omi/HtrA2间差异有统计学意义(P均<0.01)。结论腹腔注射氢气的LIR损伤兔血清TRAIL、Omi/HtrA2及骨骼肌组组织匀浆上清Omi/HtrA2表达均降低。TRAIL、Omi/HtrA2可能参与LIR的发生发展。氢气可通过抑制TRAIL的活化及血清及骨骼肌Omi/HtrA2释放,抑制细胞凋亡。
Objective gand(TRAIL)in serum,and mitochondrial serine protease(OMI/HtrA2)in serum and skeletal muscle homogenate supernatant after intraperitoneal hydrogen injection in rabbits with limb ischemia-reperfusion(LIR)injury,and to explore their possible mechanisms.Methods drogen group(group H),LIR group(group I),and control group(group C),with 15 rabbits in each group.In the groups H and I,the cuff was tied to the pulsating place of the femoral artery at the base of the right posterior limb of rabbits(blood flow could be completely blocked when the systolic blood pressure was 20-30 mmHg),and the balloon switch was opened when the cuff was tied for 4 h(reperfusion).Rabbits in the group H were intraperitoneally injected with 5 mL/kg hydrogen5 min before reperfusion,while group C and I with the same volume of air at the same time,and in the group C,we only used the same cuff to tie the same part of rabbits.Venous blood and tibialis anterior muscle tissues of big-ear white rabbits were collected at 24,72 and 168 h after reperfusion,respectively,and we detected TRAIL,Omi/HtrA2 in serum and Omi/HtrA2 in skeletal muscle tissues.Results creased in the serum of group H at 24,72 and 168 h after reperfusion(all P<0.01);compared with the group I,the level of Omi/HtrA2 in the homogenate supernatant of skeletal muscle tissues decreased in the group H at 24,72 and 168 h after reperfusion(all P<0.01).Compared with the same group at different time periods,there were statistically significant differences in serum TRAIL and skeletal muscle tissue homogenate supernate Omi/HtrA2 between the groups I and H(all P<0.01),and there was statistically significant difference in the serum Omi/HtrA2 between the rabbits in the group I(P<0.01).Conclusions nate supernatant decrease after intraperitoneal injection of hydrogen during LIR injury in rabbits.TRAIL and Omi/HtrA2 may be involved in the development and progression of LIR.Hydrogen can inhibit apoptosis by inhibiting the activation of TRAIL and the release of Omi/HtrA2.
作者
刘丹丹
李林
董云
崔昌盛
庄宝祥
田华
王岱君
LIU Dandan;LI Lin;DONG Yun;CUI Changsheng;ZHUANG Baoxiang;TIAN Hua;WANG Daijun(Weifang Medical University,Weifang 261053,China)
出处
《山东医药》
CAS
2021年第6期36-39,共4页
Shandong Medical Journal
基金
山东省医药卫生科技发展计划项目(2017WS412)
山东省自然科学基金资助项目(ZR2010HL047)。
