摘要
探究黑灵芝多糖(PSG)对脂多糖(LPS)诱导的大鼠急性肠道炎症的作用机制。本实验将大鼠随机分为正常组(Con)、模型组(LPS)、阳性对照组(DEX)和黑灵芝多糖低、中、高剂量组(PSG),每组10只。PSG低、中、高剂量组分别灌胃黑灵芝多糖20、40、80 mg/kg。Con组、LPS组与PSG组则每天灌胃等量的生理盐水,连续灌胃7 d。第7 d灌胃结束后,腹腔注射0.8 mg/kg的LPS溶液建立急性肠道炎症模型。通过观察大鼠空肠组织形态、检测细胞因子IL-1β及IL-10含量等指标,结果发现,PSG对LPS诱导的大鼠具有抗炎作用。与LPS组相比,PSG组空肠组织形态明显得到改善,肠道组织中IL-1β和IL-10含量被调节至正常水平。进一步,利用超高效液相色谱-四级杆串联飞行时间质谱(UPLC-Q-TOF/MS)方法获得了四组大鼠盲肠内容物样品的代谢轮廓图。结果发现,与Con组相比,LPS组大鼠盲肠内容物中存在22种代谢物。同时,PSG干预可回调PC(24∶0/20∶4(5Z,8Z,11Z,14Z))、DG(16∶0/20∶3(5Z,8Z,11Z)/0∶0)等12种差异代谢物,进而影响甘油磷脂代谢、亚油酸代谢和α-亚麻酸代谢等三条代谢通路,发挥其对LPS诱导大鼠的抗炎作用。综上,PSG可改善LPS所致急性肠道炎症,作用机理与其抑制促炎因子的释放和代谢紊乱有关。
The protective effects of Ganoderma atrum polysaccharide(PSG)on acute intestinal inflammation caused by lipopolysaccharide(LPS)were investigated.Rats were randomly divided into a control group(Con),a model group(LPS),positive control group(DEX)and three groups treated with PSG:Low-dose group(PSG-20),middle-dose group(PSG-40)and high-dose group(PSG-80),with 10 rats in each group.Rats were orally administered either vehicle(0.9%saline)alone or vehicle composition containing PSG(20,40,and 80 mg/kg/d)for 7 days.After intragastric administration on the 7th day,0.8 mg/kg LPS was injected intraperitoneally to establish an acute intestinal inflammation model.The results indicated that PSG has an anti-inflammatory effect on LPS-induced rats by observing jejunum histology,detecting the content of IL-1βand IL-10 in intestinal tissue.Compared with LPS group,the morphology of the jejunum tissue and the content of IL-1βand IL-10 were adjusted to normal levels.Furthermore,the metabolic effects of PSG were evaluated by screening and identifying differential metabolites and exploring metabolic pathways in rats based on UPLC-Q-TOF/MS metabolomics analysis.Accordingly,results indicated that 22 metabolites were significantly(P<0.05)changed in acute intestinal inflammation rats when compared to control rats.Meanwhile,PSG pretreatment could restore 12 different metabolites such as PC(24∶0/20∶4(5Z,8Z,11Z,14Z)),DG(16∶0/20∶3(5Z,8Z,11Z)/0∶0),and then participated in three metabolic pathways of glycerophospholipid metabolism,linoleic acid metabolism andα-linolenic acid metabolism,exerting its anti-inflammatory effects on LPS-induced rats.Altogether,PSG could improve LPS-induced acute intestinal inflammation and its mechanism were linked to inhibit release of proinflammatory mediators and reverse metabolic pathway disturbances.
作者
李露
付王威
吴睿婷
吴文英
尹术华
宋也好
万敏
李文娟
LI Lu;FU Wangwei;WU Ruiting;WU Wenying;YIN Shuhua;SONG Yehao;WAN Min;LI Wenjuan(College of Food Science,Nanchang University,Nanchang 330047,China;Food Inspection and Testing Institute of Jiangxi Province,Nanchang 330001,China)
出处
《食品工业科技》
CAS
北大核心
2021年第6期310-317,共8页
Science and Technology of Food Industry
基金
国家自然科学基金地区项目(81860713)
南昌大学研究生创新项目(CX2018116)。
关键词
黑灵芝多糖
急性肠道炎症
超高效液相色谱-四级杆串联飞行时间质谱
代谢组学
抗炎
Ganoderma atrum polysaccharide
acute intestinal inflammation
ultra-performance liquid chromatography-quadrupole time-of-flight mass spectrometry
metabolomics
anti-inflammatory
