摘要
目的探究牛蒡甙元对老年大鼠肾缺血再灌注损伤模型的影响。方法建立老年大鼠肾缺血再灌注损伤模型,采用随机数字表法将大鼠随机分为6组:假手术组(Sham组)、牛蒡甙元组(ACR组)、肾缺血再灌注模型组(RIRI组)、RIRI+ACR(10 mg/kg)组、RIRI+ACR(20 mg/kg)组和RIRI+ACR(50 mg/kg)组进行后续实验。试剂盒检测血肌酐(Scr)、尿素氮(BUN)水平;苏木精-伊红(HE)染色检测肾组织病理损伤;末端脱氧核苷酸转移酶介导的dUTP缺口末端标记测定法(TUNEL)染色检测细胞凋亡;酶联免疫吸附测定(ELISA)检测炎症因子肿瘤坏死因子-α(TNF-α)、白细胞介素-6(IL-6)、白细胞介素-8(IL-8)水平;蛋白免疫印迹检测肾损伤分子-1(KIM-1)、中性粒细胞明胶酶相关脂质运载蛋白(NGAL)、cleaved caspase-3、cleaved caspase-9、胞内磷脂酰肌醇激酶(PI3K)、p-PI3K、蛋白激酶B (Akt)、p-Akt蛋白表达水平。加入PI3K/Akt通路抑制剂LY294002后,将大鼠随机分为5组:Sham组、RIRI组、RIRI+ACR(50 mg/kg)组、RIRI+LY294002组和RIRI+ACR+LY294002组。Western blot检测各组大鼠PI3K、p-PI3K、Akt、p-Akt蛋白表达水平;试剂盒检测Scr、BUN水平;ELISA检测TNF-α、IL-6、IL-8水平。结果与RIRI组相比较,RIRI+ACR(20、50 mg/kg)组大鼠Scr、BUN水平降低,病理损伤程度改善,KIM-1、NGAL蛋白水平降低,凋亡细胞数减少,cleaved caspase-3、cleaved caspase-9蛋白水平降低,TNF-α、IL-6、IL-8水平降低,p-PI3K/PI3K、p-Akt/Akt比值升高。结论牛蒡甙元对老年大鼠肾缺血再灌注损伤具有保护作用。
Objective To explore the effect of Arctigenin on renal ischemia-reperfusion injury model in aged rats. Methods The model of renal ischemia-reperfusion injury in elderly rats was established, and the rats were randomly divided into 6 groups by random number table method: Sham group, Arctigenin group(ACR), renal ischemia-reperfusion model group(RIRI), RIRI+ACR(10 mg/kg) group, RIRI+ACR(20 mg/kg) group and RIRI+ACR(50 mg/kg) group for follow-up experiments. Kit was used to detect Scr and BUN levels. HE staining was used to detect pathological damage of renal tissue. Apoptosis was detected by TUNEL staining. The levels of inflammatory cytokines TNF-α, IL-6 and IL-8 were detected by ELISA. The expression levels of KIM-1, NGAL, cleaved caspase-3, cleaved caspase-9, PI3 K, p-PI3 K, Akt and p-Akt were detected by Western blot. After the PI3 K/Akt pathway inhibitor LY294002 was added, the rats were randomly divided into 5 groups: Sham group, RIRI group, RIRI+ACR(50 mg/kg) group, RIRI+LY294002 group and RIRI+ACR+LY294002 group. Western blot was used to detect the expression levels of PI3 K, p-pi3 k, Akt and p-akt in the rats in each group. The kit was used to detect the levels of Scr and BUN, and ELISA was used to detect the levels of TNF-α, IL-6 and IL-8. Results The results showed that compared with RIRI group, RIRI+ACR(20, 50 mg/kg) group of Scr and BUN levels in rats decreased obviously, obviously improved pathological damage degree, KIM-1, NGAL protein levels significantly decreased, apoptotic cells significantly reduced, cleaved caspase-3, cleaved caspase-9 protein levels significantly decreased, the TNF alpha, IL-6, IL-8 levels significantly decreased, p-PI3 K/PI3 K, p-Akt/Akt ratio increased significantly. Conclusion Arctigenin has protective effect on renal ischemia-reperfusion injury in aged rats.
作者
柯筱玥
黄梦娇
杜恒健
刘尧
朱颖
方靖
韩雪娇
Ke Xiaoyue;Huang Mengjiao;Du Hengjian(Dept of Geriatrics,Sichuan Academy of Medical Sciences·Sichuan People’s Hospital,Chengdu 610027)
出处
《安徽医科大学学报》
CAS
北大核心
2021年第1期96-102,共7页
Acta Universitatis Medicinalis Anhui
基金
四川省卫生和计划生育委员会科研课题(编号:16PJ363)。
