摘要
目的:研究前列腺素E1(PGE1)联合缺血预处理对肾部分切除术肾缺血再灌注损伤的防治效应及其分子机制,为临床防治肾部分切除术肾缺血再灌注损伤提供实验依据。方法:构建肾部分切除术肾缺血再灌注损伤模型,随机分为假手术组、缺血再灌注组、缺血预处理组、PGE1组及PGE1联合缺血预处理组。病理检测肾小管损伤程度;酶联免疫吸附法(ELISA)检测血清细胞间黏附分子-1(intercellular cell adhesion molecule-1,ICAM-1)、肾损伤分子1 (kidney injury molecule-1,Kim-1)和血栓调节蛋白(thrombomodulin,TM)水平;实时荧光定量PCR检测肾组织Kim-1和TM mRNA表达水平。结果:与缺血再灌注模型组比较,前列腺素E1联合预处理对肾部分切除术肾缺血再灌注肾小管损伤最低;与PGE1组或缺血预处理组比较,前列腺素E1联合缺血预处理Cr水平和Kim-1水平最低,然而差异无统计学意义(P>0.05)。与ELISA结果一致,与PGE1组或缺血预处理组比较,前列腺素E1联合缺血预处理组Kim-1的表达水平最低,但差异无统计学意义(P>0.05)。结论:PGE1联合缺血预处理在改善肾缺血再灌注损伤中具有一定协同增效作用,其机制可能部分与下调Kim-1表达相关。
Objective: To investigate early protective effect of combination of PGE1 and pretreatment in rat with renal ischemia reperfusion injury (IRI)and explore its mechanism and provide a new idea for treatment. Methods:Renal Ischemia reperfusion injury models in rats were constructed by ligating bilateral renal arteries for 45min. The rats were randomly divided into five group, sham-operated control group, IRI control group, PGE1 treated groups, pretreated group and combination of PGE1 and pretreatment. Renal pathologic changes were observed by HE staining;The production of Cr, ICAM- 1,Kim-1 and TM in serum were detected by Enzyme linked immunosorbent assay(ELISA);Kim-1 and TM mRNA expression in renal tissue were examined by real time polymerase chain reaction(RT-PCR). Results:Compared with IRI control group, the renal pathologic injury was decreased in combination of PGE1 and pretreatment group.Combination of PGE1 and pretreatment significantly decreased Cr and Kim-1 production (P<0.05);Consist ent with ELISA results,compared with PGE1 and pretreatment group, Kim- 1 mRNA expression was lowest in Combination treated group, but there was no statistical significance(P>0.05). Conclusion: The results indicated that Combination of PGE1 and pretreatment improve effect and decrease toxicity in IRI model and the mechanisms may relate to partially down-regulated Kim-1. This study will provide an idea and experimental basic for treating renal alchemist-reperfusion injury.
作者
曹先德
郑慧敏
万春平
CAO Xiande;ZHENG Huimin;WAN Chunping(Department of Urinary Surgery, the Affiliated Hospital of Jining Medical Univeristy,Shandong272029;Central Laboratory, the First Affiliated Hospital of Yunnan University of Traditional Chinese Medicine)
出处
《交通医学》
2019年第2期119-122,125,共5页
Medical Journal of Communications
基金
山东省高等学校科技计划项目(J14LL05)
