摘要
目的整合发掘更多冠心病血瘀证的标志基因,研究已知冠心病血瘀证差异基因的生物机理,构建关键蛋白互作模块与miRNA-mRNA调控网络,丰富靶点间的相互作用网络,并分析其核心生物过程与通路。方法利用中国知网、万方、维普、中国生物医学文献、Embase、PubMed六个数据库获取冠心病血瘀证相关基因文献;按照纳排标准筛选符合要求的文献提取数据,运用Metascape数据库针对全部差异mRNA构建蛋白质间互相作用的网络模块,并对上调mRNA和下调mRNA的通路进行富集和功能注释。利用starBase数据库预测冠心病血瘀证相关miRNA的靶基因,并与文献整合的差异mRNA相互映射,运用OmicShare Tools绘制冠心病血瘀证相关miRNA-mRNA调控网络。结合Webgestalt在线工具,针对miRNAmRNA调控网络中相关mRNA进行KEGG和GO富集分析。结果研究纳入冠心病血瘀证相关基因文献15篇,获得113个mRNA(66个上调、47个下调),101个miRNA(55个上调、46个下调)。研究通过蛋白互作网络得到2组关键蛋白:①IL6、EGR1、TP53、JUN、FOS;②CTSL、HLA-DRB1、HLA-DQB1、HLA-DRB5。构建得到miRNA-mRNA调控网络的关键节点,包括24个miRNA(10个上调、14个下调)和22个mRNA(10个上调、22个下调),其中10个节点与部分纳入文献的研究结果吻合。结论冠心病血瘀证涉及多种生物功能、多条信号通路,其miRNA-mRNA调控网络机制可能与炎症和免疫反应、凝血功能、细胞分化与凋亡等较为相关。
Objective To explore more marker genes in blood stasis syndrome of coronary heart disease, study the biological mechanism of known differential genes in blood stasis syndrome of coronary heart disease, construct key protein interaction modules and miRNA-mRNA regulatory network, enrich the interaction between targets Network, and analyze its core biological processes and pathways. Methods Genetic literature related to blood stasis syndrome of coronary heart disease was retrieved from 6 databases including CNKI, Wan Fang, VIP, SinoMed, Embase, and PubMed.The data were extracted from the qualified literature according to the inclusion and exclusion criteria. The Metascape database was used to construct protein interaction network modules for all differential mRNAs, and to enrich pathways and functional annotations of up and down regulated mRNA. The starBase database was used to predict the target genes of miRNA related to blood stasis syndrome of coronary heart disease, and the differential mRNA integrated with the literature was mapped to each other. The OmicShare Tools was used to draw the miRNA-mRNA regulatory network related to blood stasis syndrome of coronary heart disease. Webgestalt online tool was used to conduct KEGG and GO enrichment analysis on the relevant mRNA in the miRNA-mRNA regulatory network. Results The study included 15 pieces of literature on genes related to the coronary heart disease with blood stasis syndrome and obtained 113 mRNAs(66 up-regulated and 47 down-regulated) and 101 miRNAs(55 up-regulated and 46 down-regulated). The study obtained 2 sets of key proteins through the protein interaction network: ①IL6, EGR1, TP53, JUN, FOS;②CTSL, HLADRB1, HLA-DQB1, HLA-DRB5. The key nodes in the miRNA-mRNA regulatory network include 24 miRNAs(10 upregulated and 14 down-regulated) and 22 mRNAs(10 up-regulated and 22 down-regulated), of which 10 nodes are consistent with some of the results of the included literature. Conclusion Blood stasis syndrome of coronary heart disease involves multiple bio
作者
谌子诺
王阶
陈恒文
刘咏梅
何浩强
陈光
杨光
周思远
Shen Zinuo;Wang Jie;Chen Hengwen;Liu Yongmei;He Haoqiang;Chen Guang;Yang Guang;Zhou Siyuan(College of Clinical Medicine,Beijing University of Chinese Medicine,Beijing 100029,China;Guang'anmen Hospital,China Academy of Chinese Medical Sciences,Beijing 100053,China)
出处
《世界科学技术-中医药现代化》
CSCD
北大核心
2020年第11期3872-3881,共10页
Modernization of Traditional Chinese Medicine and Materia Medica-World Science and Technology
基金
国家自然科学基金委员会面上基金项目(81974556):冠心病稳定型心绞痛痰瘀互结证多组学网络模块整合研究,负责人:王阶
国家中医药管理局中医药传承与创新“百千万”人才工程(岐黄工程),负责人:王阶
中华人民共和国科学技术部国家重点研发计划(2020YFC2002701):亚健康状态的中医辨证研究,负责人:潘菊华。
