摘要
目的调查中国50岁以上表观健康人群脂蛋白相关磷脂酶A2(Lp-PLA2)水平分布,建立相应参考范围,并进行性别和地区差异分析。方法全国北方和南方大部分地区的24个中心收集50岁以上表观健康人群7 897份标本,排除信息缺失和离群值样本后共计7 084份标本纳入统计分析,其中来自北方区和南方区的受试者分别为4 752和2 332名。年龄50~95(63.8±10.0)岁。男性受试者4 029名,年龄(64.8±10.3)岁;女性受试者3 055名,年龄(62.6±9.4)岁。空腹状态下采集外周静脉样本,使用全自动磁微粒化学发光法检测Lp-PLA2质量浓度。依据中华人民共和国卫生行业标准WS/T 402-2012和CLSI C28-A3文件进行总体和亚组间参考区间分析,采用Z检验确定是否按照性别和地区设置参考区间。结果 50岁以上表观健康人总体、男性、女性的Lp-PLA2质量浓度分布均为正态分布,分别为(143.9±52.6)、(149.0±53.7)和(137.2±50.3)μg/L,95%参考上限分别为230.4 μg/L(90%CI 228.6~232.1 μg/L)、237.3 μg/L(90%CI 234.9~239.7 μg/L)和219.9 μg/L(90%CI 217.3~222.5 μg/L)。但性别间Z值(9.6)<Z*(16.3)截断值,提示男性和女性可采用同一95%参考区间。北方地区和南方地区50岁以上表观健康人Lp-PLA2水平差异无统计学意义(P=0.12)。结论通过数据调查分析并建立了50岁以上表观健康人群统一的Lp-PLA2质量浓度95%参考区间。男性Lp-PLA2质量浓度略高于女性,但是不符合分组设置参考区间标准。同样南方与北方地区间Lp-PLA2平均质量浓度无差异。
Objective To investigate the distribution range of lipoprotein-associated phospholipase A2(Lp-PLA2)levels in apparent healthy people aged over 50 years,establish a corresponding reference interval,and analyze differences between genders and regions.Methods A total of 7897 fasting venous blood samples were collected from 24 centers from apparent healthy people,and 7084 samples were included in subsequent statistical analysis after excluding samples with missing information and outliers.Four thousand seven hundred and fifty-two samples and 2332 samples were collected from the northern and southern China,respectively.The average age was(63.8±10.0)years old in this cohort.The average age of 4029 males and 3055 females were(64.8±10.3)and(62.6±9.4)years old,respectively.Automatic magnetic particle chemiluminescence method was used to detect Lp-PLA2 levels.The analysis of overall and subgroup reference interval was conducted according to the documents of the People′s Republic of China Health Industry Standard WS/T 402-2012 and CLSI C28-A3.Results The distribution of Lp-PLA2 levels in apparent healthy people aged over 50 years follows a normal distribution among all participants,male or female population,the average levels were(143.9±52.6),(149.0±53.7)and(137.2±50.3)μg/L,respectively.The 95%reference upper limit is 230.4μg/L(90%CI 228.6-232.1μg/L),237.3μg/L(90%CI 234.9-239.7μg/L)and 219.9μg/L(90%CI 217.3-222.5μg/L),respectively.The Z value between genders(9.6)was<Z*(16.3)cut-off value,suggesting that the same 95%reference interval can be used for both men and women.The average level of Lp-PLA2 form apparent healthy people aged over 50 years is similar between the northern and southern regions(P=0.12).Conclusions Present study established a unified 95%reference interval of Lp-PLA2 levels for apparent healthy people aged over 50 years through large cohort investigation and analysis.Although the Lp-PLA2 levels is slightly higher in males than in females,the same 95%reference interval can be used for both men
作者
蔺亚晖
龚美亮
张鹏
邓新立
于金星
郑磊
周洲
丛玉隆
Lin Yahui;Gong Meiliang;Zhang Peng;Deng Xinli;Yu Jinxing;Zheng Lei;Zhou Zhou;Cong Yulong(Center of Laboratory Medicine,Beijing Key Laboratory for Molecular Diagnostics of Cardiovascular Diseases,Chinese Academy of Medical Sciences&Peking Union Medical College,National Center for Cardiovascular Diseases,Beijing 100037,China;Center of Clinical Laboratory,Second Medical Center of Chinese PLA General Hospital,Beijing 100853,China;Laboratory Medicine Department,Nanfang Hospital Southern Medical University,Guangzhou 510515,China)
出处
《中华检验医学杂志》
CAS
CSCD
北大核心
2021年第1期32-38,共7页
Chinese Journal of Laboratory Medicine
基金
国家卫生健康委员会老龄健康司项目(2019年)
军委后勤保障部卫生局保健专项课题(17BJZ45)。
关键词
老年人
脂蛋白相关磷脂酶A2
参考区间
Aged
Lipoprotein-associated phospholipase A2
Reference interval
