摘要
目的:探讨桂枝汤桂枝-白芍不同比例配伍对盐敏感高血压大鼠心脏交感神经过度激活的影响及其作用机制。方法:将40只6周龄雄性盐敏感大鼠随机分为5组,空白组、模型组、桂枝汤组(桂枝-白芍1∶1组、桂枝-白芍1∶2组和桂枝-白芍2∶1组)每组8只,空白组给予低盐饲料饲养,其余4组给予8%高盐饲料饲养,饲养4周后灌胃,空白组、模型组大鼠每天给予2 mL·kg^(-1)生理盐水灌胃,桂枝-白芍1∶1组、桂枝-白芍1∶2组和桂枝-白芍2∶1组每天分别按4.0,5.5,5.5 g·kg^(-1)给予桂枝汤水煎剂灌胃,连续干预4周。IITC多通道无创血压计检测大鼠治疗前后收缩压变化;超声心动图检测左室前壁舒张末期厚度(LVAWd)和室间隔舒张期厚度(IVSd)以反应心肌肥厚程度;苏木素-伊红(HE)染色和马松(Masson)染色观察大鼠心肌形态学变化;蛋白免疫印迹法(Western blot)检测各组大鼠心肌组织神经生长因子(NGF),生长相关蛋白43(GAP43)和酪氨酸羟化酶(TH)蛋白的表达。结果:桂枝汤干预4周后,与空白组比较,模型组大鼠血压,LVAWd和IVSd显著升高,NGF,TH和GAP43蛋白表达显著增高(P<0.01);HE和Masson染色结果显示,模型组大鼠心肌细胞水肿,大量炎性细胞浸润,心肌纤维大量增生且排列紊乱,细胞间质可见大量胶原沉积。与模型组比较,桂枝汤各组大鼠收缩压上升缓慢,NGF,TH和GAP43蛋白表达明显降低(P<0.05,P<0.01),以桂枝-白芍1∶1组效果最佳;超声心动图结果显示,与模型组比较,桂枝-白芍1∶1组可降低LVAWd和IVSd(P<0.05,P<0.01);桂枝-白芍1∶2组可明显降低IVSd(P<0.05),但LVAWd与模型组比较,差异无统计学意义;桂枝-白芍2∶1组LVAWd和IVSd均无统计学差异;心肌形态学方面,桂枝汤各组较模型组均可减轻细胞水肿和炎性细胞浸润,减少心肌纤维增生和胶原沉积,改善心肌纤维排列紊乱,其中以桂枝汤桂枝-白芍1∶1组效果最佳。结论:桂枝汤可抑制心脏交感�
Objective: To explore the effect and mechanism of Guizhitang with different proportions of Cinnamomi Ramulus and Paeoniae Alba Radix on overactive cardiac sympathetic nerves in salt-sensitive hypertensive rats. Method: Randomly divide the forty male 6-week-old salt-sensitive hypertensive rats into five groups:the normal control group,the model group,the Cinnamomi Ramulus and Paeoniae Alba Radix 1∶1 group,the Cinnamomi Ramulus and Paeoniae Alba Radix 1∶2 group,and the Cinnamomi Ramulus and Paeoniae Alba Radix 2∶1 group,each group has 8 animals,the normal control group was fed with low-salt feed,and the remaining four groups were fed with 8% high-salt feed. After 4 weeks of feeding,gastric feeding was started.Give both the normal control group and model group saline and the Cinnamomi Ramulus and Paeoniae Alba Radix 1∶1 group,the 1∶2 group,and the 2∶1 group,were given Guizhitang aqueous solution at 4.0,5.5 and5.5 g·kg^(-1),respectively. Continuous gavage intervention was held for 4 weeks. IITC multi-channel non-invasive sphygmomanometer was used to detect changes of systolic blood pressure before and after treatment in rats. Left ventricular anterior wall end-diastolic thickness(LVAWd)and interventricular septal diastolic thickness(IVSd)were detected by echocardiography. Hematoxylin-eosin(HE)staining and Masson staining were used to observe the myocardial morphological changes of rats in each group,Western blot was used to detect the expression of nerve growth factor(NGF),growth-associated protein 43(GAP43)and tyrosine hydroxylase(TH)protein.Result: After 4 weeks of intervention with Guizhitang,compared with the normal control group,the blood pressure,LVAWd and IVSd of the model group were significantly increased,and the expressions of NGF,TH and GAP43 protein were significantly increased(P<0.01). HE and Masson staining results showed that the model group had myocardial cell edema,a large number of inflammatory cell infiltration,myocardial fiber hyperplasia and disordered arrangement, and a large amount
作者
韩晓伟
王永成
马度芳
李媛
董峰
赵恒懿
姜萍
陈文静
陈纪烨
迟辰昱
李晓
HAN Xiao-wei;WANG Yong-cheng;MA Du-fang;LI Yuan;DONG Feng;ZHAO Heng-yi;JIANG Ping;CHEN Wen-jing;CHEN Ji-ye;CHI Chen-yu;LI Xiao(Shandong University of Traditional Chinese Medicine(TCM),Ji'nan 250014,China;Affiliated Hospital of Shandong University of TCM、Ji'nan 250014,China)
出处
《中国实验方剂学杂志》
CAS
CSCD
北大核心
2021年第1期81-87,共7页
Chinese Journal of Experimental Traditional Medical Formulae
基金
国家自然科学基金项目(81673970)
国家中医药管理局第四批全国中医(临床、基础)优秀人才研修项目(国中医药人教发[2017]24)
山东张氏风湿病清消流派传承工作室项目。
关键词
桂枝汤
高血压
神经生长因子
交感神经系统
心肌损害
Guizhitang
hypertension
nerve growth factor
sympathetic nervous system
myocardial damage
