摘要
目的通过盲肠不同部位结扎穿孔致脓毒症模型的研究,探索符合临床病理过程的脓毒症模型。方法50只KM雄性6周龄小鼠分为5组,空白对照(NC)组、假手术(Sham)组、轻度脓毒症(Min)组、中度脓毒症(Mod)组和重度脓毒症(Sev)组。Sham组仅进行开腹、关腹程序;Min组、Mod组和Sev组以盲肠结扎穿孔术处理,分别结扎盲肠1/3、1/2、2/3(从盲肠远端起至回盲瓣)后,建立脓毒症模型。评估并比较24 h内小鼠临床症候、生存状况及死亡率。同时,解剖小鼠肝脏及盲肠组织,苏木素-伊红(HE)染色观察形态变化。24 h后进行小鼠眼眶取血,检测炎性因子水平,包括肿瘤坏死因子-α(TNF-α)、白细胞介素-6(IL-6)。结果Min组小鼠死亡率达20%,Mod组达40%,Sev组达90%。HE染色显示肝脏及盲肠细胞肿胀,组织毛细血管充血,被炎性细胞浸润,肝脏大量空泡脂肪样变性,且病变程度Sev组最重,其次为Mod组,Min组最轻。Mod组TNF-α、IL-6水平较NC组明显升高(P<0.05)。结论Mod模型是符合临床的1种理想动物模型。
Objective Through the study of septic models caused by ligation and perforation in different parts of cecum,a clinicopathological sepsis model was explored.Methods Fifty 6-week-old km male mice were divided into five groups,the namely blank control(NC)group,the Sham operation(Sham)group,the minor sepsis(Min)group,the moderate sepsis(Mod)group and the severe sepsis(Sev)group.Only laparotomy and abdomen-closing procedures were performed in the Sham group.The Min,Mod and Sev group were treated with cecal ligation and puncture.After ligation of the cecum 1/3,1/2 and 2/3(from the distal cecum to the ileocecal valve),the sepsis model was established.The clinical symptoms,survival status and mortality of the mice within 24 h were subsequently graded and compared.After 24 h,orbital blood was taken,and the expression levels of tumor necrosis factor-α(TNF-α)and interleukin-6(IL-6)were measured.Liver and cecum tissues were dissected,and hematoxylin-eosin(HE)staining was performed to observe morphological changes.Results The mortality rate of mice in the Min group was 20%.The Mod group had a mortality rate of 40%.The mortality rate of the Sev group was 90%.HE staining showed swelling of liver and cecum cells,hyperemia of tissue capillaries,infiltration by inflammatory cells,massive vacuolated steatosis of liver,and the degree of lesion was the highest in the Sev group,followed by the Mod group,and the lowest in the Min group.The levels of TNF-αand IL-6 in the Mod group were significantly different(P<0.05).Conclusion The moderate sepsis model is an ideal animal model for clinical compliance.
作者
王圆圆
陈晴
赵晗
王婧蓉
何长江
叶小利
WANG Yuanyuan;CHEN Qing;ZHAO Han;WANG Jingrong;HE Changjiang;YE Xiaoli(School of Life Science,Southwest University,Chongqing 400715,China)
出处
《重庆医学》
CAS
2021年第4期552-556,共5页
Chongqing medicine
基金
中央高校基本科研业务费(XDJK2019D026)。
