摘要
目的:探讨膝痹病(膝关节骨性关节炎)中医证候分型与关节软骨中MMP1,MMP9,MMP13及TIMP2阳性表达的相互关系。方法:膝痹病(膝关节骨性关节炎)手术(关节镜和关节置换)患者90例,按中医学证候分型标准分成风湿热痹证组、风寒湿痹证组、肝肾亏虚证组、瘀血痹阻证组共4组。免疫组织化学染色法测定关节软骨中400倍镜下MMP1,MMP9,MMP13及TIMP2阳性细胞数。结果:各证型组400倍镜下关节软骨中MMP1,MMP9,MMP13及TIMP2阳性细胞数比较,差异有统计学意义(P<0.05)。结论:膝痹病患者术后关节软骨中MMP1,MMP9,MMP13及TIMP2的阳性表达可以为膝痹病中医宏观辨证分型提供参考依据。
Objective:To explore the correlation between TCM syndrome classification and positive expression of MMP1,MMP9,MMP13,and TIMP2in patients’articular cartilage with knee osteoarthritis.Methods:90patients with osteoarthritis of the knee who underwent surgery of arthroscopic knee debridement or knee joint replacement were divided into rheumatic heat arthralgia syndrome group,wind cold damp arthralgia syndrome group,liver-kidney deficiency syndrome group and blood stasis and arthralgia syndrome group according to standard of TCM syndrome types.Intraoperative articular cartilages were obtained for immune-histochemical staining with MMP1,MMP9,MMP13,and TIMP2.The number of MMP1,MMP9,MMP13and TIMP2positive cells in articular cartilage were determined under 400×microscope.Results:Comparison of the number of MMP1,MMP9,MMP13and TIMP2positive cells in articular cartilage under 400×microscope in each syndrome group showed statistically significant difference(P<0.05).Conclusion:The positive expression of MMP1,MMP9,MMP13and TIMP2in the postoperative articular cartilage of patients with knee osteoarthritis can be used as the reference basis for the macroscopic syndrome differentiation of TCM.
作者
胡华
李秀成
周刚
陈茂华
杨桂英
HU Hua;LI Xiucheng;ZHOU Gang;CHEN Maohua;YANG Guiying(Traditional Chinese Medicine Hospital of China Three Gorges University(Yichang Hospital of Traditional Chinese Medicine),Yichang 443008,Hubei China.)
出处
《中国中医骨伤科杂志》
CAS
2021年第1期15-18,23,共5页
Chinese Journal of Traditional Medical Traumatology & Orthopedics
基金
宜昌市卫生科研课题(A19-301-45)
湖北省自然科学基金面上项目(2019CFB560)。
关键词
膝骨关节炎
关节软骨
中医证型
金属基质蛋白酶
金属基质蛋白酶抑制剂
knee osteoarthritis
articular cartilage
TCM syndrome type
matrix metalloproteinase
tissue inhibitor of metalloproteinases
