摘要
目的通过优化人参多糖(Ginseng Polysaccharide)的提取工艺,探讨人参多糖的体外抗氧化能力.方法应用水提醇沉法,在料液比、提取温度、提取时间、提取次数单因素试验的基础上,采用4因素3水平正交分析法优化人参多糖的提取工艺,同时对人参多糖的总抗氧化能力(FRAP法)、清除1,1-二苯基苦基苯肼自由基(DPPH·)和羟基自由基(·OH)的能力进行测定.结果影响多糖得率的顺序依次为提取温度>料液比>提取时间>提取次数,此研究确定了人参多糖的最优提取工艺:提取温度60℃,料液比m(g)∶V(mL)=1∶30,提取时间60 min,提取次数2次,在此条件下获得的最高得率为(9.87±0.35)%.人参多糖清除DPPH自由基的IC50值为7.58μg/mL,清除羟自由基的IC50值为145.72μg/mL.体外抗氧化结果表明:人参多糖的总抗氧化能力、清除DPPH自由基和羟自由基的能力均强于相同浓度下水溶性维生素E,且呈剂量依赖性增加.结论人参多糖具有较好的体外抗氧化能力.
Objective The antioxidant capacity of Ginseng polysaccharide in vitro was studied by optimizing the extraction process of Ginseng polysaccharide.Method By the method of water extract-alcohol precipitation,on the basis of single factor experiment,ratio of materials to liquid,extraction temperature,extraction time,extraction times,extraction process of Ginseng polysaccharide was optimized by using the method of four factors three levels orthogonality analysis,the total antioxidant capacity of Ginseng polysaccharides(FRAP),1,1-diphenyl bitter scavenger phenylhydrazine free radical(DPPH·)and the ability of the hydroxyl free radical(·OH)were measured.Results The influence was concerned on polysaccharide yield,the order of parameters was extraction temperature,ratio of materials to liquid,extraction time and extraction times.The optimal process for extracting polysaccharides from Ginseng was determined as extraction temperature 60℃,ratio of materials to liquid 1∶30 g/mL,extraction time was 60 min and 2 extraction times.The highest yield was(9.87±0.35)%.The IC50 value of DPPH free radical scavenging was 7.58μg/mL,hydroxyl radical scavenging was 145.72μg/mL by Ginseng polysaccharide.In vitro antioxidant results showed that the total antioxidant capacity and scavenging ability of DPPH free radicals and hydroxyl free radical were stronger than those of water soluble VE at the same concentration and increased in dose-dependent manner.Conclusion Ginseng polysaccharide has good antioxidant capacity in vitro.
作者
葛俊宏
许梦然
王迦琦
高婧雯
侯俊宇
李明慧
李坦
申野
孙新
GE Junhong;XU Mengran;WANG Jiaqi;GAO Jingwen;HOU Junyu;LI Minghui;LI Tan;SHEN Ye;SUN Xin(Pharmacy School of Beihua University,Jilin 132013,China;Jilin Provincial Laboratory of Molecular Geriatrics,Jilin 132013,China;Basic Medical College of Beihua University,Jilin 132013,China)
出处
《北华大学学报（自然科学版）》
CAS
2021年第1期36-41,共6页
Journal of Beihua University（Natural Science）
基金
吉林省中医药管理局基金项目(2019131)
吉林省科技厅重点实验室研究项目(20200201178JC).
关键词
人参多糖
提取工艺
抗氧化活性
Ginseng polysaccharide
extraction process
antioxidant activity
