摘要
目的建立红景天破壁饮片(UGP)、破壁粉体(UP)及传统饮片(DP)的HPLC指纹图谱及其分析方法,为全面评价红景天破壁饮片的质量提供参考。方法通过HPLC采集10批次红景天传统饮片、破壁粉体及破壁饮片的指纹图谱,并对其进行相似度评价、主成分分析(PCA)和系统聚类分析(HCA)。结果建立了红景天破壁饮片、破壁粉体和传统饮片的HPLC指纹图谱,确定了18个共有峰;同一批次的红景天破壁饮片、破壁粉体及其传统饮片之间的指纹图谱相似度达0.99以上;通过PCA和HCA分析,可将不同产地红景天样品分为3类。结论所建立的指纹图谱分析方法稳定、可靠、重复性好,可为红景天破壁饮片的质量控制及评价提供参考依据。
Objective To establish HPLC fingerprints of ultrafine granular powder(UGP),ultrafine powder(UP) and decoction pieces(DP) of Rhodiolae Crenulatae Radix et Rhizoma(Rhodiola) for providing reference for quality evaluation of UGP of Rhodiola.Methods Collected 10 batches of DP,UP,UGP of Rhodiola fingerprints by HPLC.And analyze the fingerprints by similarity evaluation,principal component analysis(PCA)and hierarchical cluster analysis(HCA).Results HPLC fingerprints of UGP,UP and DP of Rhodiola are established,and 18 common peaks are marked.The similarity of the same batch of UGP,UP and DP of Rhodiola are all more than 0.99.Rhodiola samples from different habitats could be divided into three categories by?PCA and HCA.Conclusion The established fingerprint analysis method is stable,reliable and repeatable.It can provide reference for the quality control and evaluation of UGP of Rhodiola combined.
作者
方碧烟
甘均龙
史军杰
彭丽华
陈炜璇
成金乐
FANG Bi-yan;GAN Jun-long;SHI Jun-jie;PENG Li-hua;CHEN Wei-xuan;CHENG Jin-le(Research Center of Chinese Herbal Resource Science and Engineering,Guangzhou University of Chinese Medicine,Guangzhou 510006,China;Key Laboratory of Technologies and Applications of Ultrafine Granular Powder of Herbal Medicine,State Administration of Traditional Chinese Medicine Zhongshan Zhongzhi Pharmaceutical Group Co.,Ltd.,Zhongshan 528437,China;Key Laboratory of Chinese Medicinal Resource from Lingnan(Guangzhou University of Chinese Medicine),Ministry of Education,Guangzhou 510006,China;Joint Laboratory of National Engineering Research Center for the Pharmaceutics of Traditional Chinese Medicines,Guangzhou 510006,China)
出处
《现代中药研究与实践》
CAS
2021年第1期33-37,共5页
Research and Practice on Chinese Medicines
基金
中山市中药破壁饮片技术与应用协同创新中心(2016C1014)。
关键词
红景天
传统饮片
破壁粉体
破壁饮片
指纹图谱
不同产地
Rhodiolae Crenulatae Radix et Rhizoma
decoction pieces
ultrafine powder
ultrafine granular powder
fingerprint
different habitats
