摘要
目的:研究异丙酚通过糖原合成酶激酶-3β(GSK-3β)/β-连环素信号(β-catenin)通路对大鼠认知功能及海马神经元凋亡的影响。方法:选择7日龄的雄性SD大鼠30只,按照随机数字表法分为对照组、异丙酚组、异丙酚+SB216763组,每组10只。对照组给予生理盐水腹腔注射,异丙酚组给予75 mg/kg异丙酚腹腔注射,异丙酚+SB216763组给予10 mg/kg的GSK-3β抑制剂SB216763尾静脉注射及75mg/kg异丙酚腹腔注射。采用Morris水迷宫检测大鼠认知功能,TUNEL试剂盒检测海马的神经元凋亡率,Western blot检测海马中相关基因的表达水平。结果:异丙酚组的逃逸潜伏期、寻找平台路径长度均长于对照组,探索时间、穿越平台次数均短于对照组;异丙酚+SB216763组的逃逸潜伏期、寻找平台路径长度均短于异丙酚组,探索时间、穿越平台次数均显著长于异丙酚组(P<0.05)。异丙酚组神经元凋亡率及GSK-3β、Bax、Caspase-3相对表达水平明显高于对照组,β-catenin、Bcl-2的相对表达水平明显低于对照组(P<0.05);而异丙酚+SB216763组的神经元凋亡率及GSK-3β、Bax、Caspase-3相对表达水平明显低于异丙酚组,β-catenin、Bcl-2相对表达水平明显高于异丙酚组(P<0.05)。结论:异丙酚通过GSK-3β/β-catenin通路影响大鼠认知功能及海马神经元凋亡。
Objective:To study the effects of propofol anesthesia on cognitive function and apoptosis of hippocampal neurons in rats through glycogen synthase kinase-3β(GSK-3β)/β-catenin signaling pathway.Methods:307-day-old male SD rats were randomly divided into a control group,a propofol group and a propofol+SB216763 group.The control group was given saline intraperitoneal injection,the propofol group was given 75 mg/kg propofol intraperitoneal injection,and the propofol+SB216763 group was given 10 mg/kg GSK-3βinhibitor SB216763 intravenous injection and 75 mg/kg propofol intraperitoneal injection.Morris water maze was used to detect their cognitive function,TUNEL kit was used to detect the neuronal apoptosis rate in hippocampus,and Western blot was used to detect the related gene expression level in hippocampus.Results:The escape latency and the length of searching in the propofol group were significantly higher than those in the control group,and the time in target quadrant and the times in platform crossing were significantly lower than those in the control group;the escape latency and the length of searching in the propofol+SB216763 group were significantly lower than those in the propofol group,and the time in target quadrant and the times in platform crossing were significantly higher than those in the propofol group(P<0.05).The apoptotic rate of neurons and the relative expression levels of GSK-3β,Bax and Caspase-3 in the propofol group were significantly higher than those in the control group,while the relative expression levels ofβ-catenin,Bcl-2 were significantly lower than those in the control group(P<0.05).The apoptotic rate of neurons and the relative expression levels of GSK-3β,Bax and Caspase-3 in the propofol+SB216763 group were significantly lower than those in the propofol group,while the relative expression levels ofβ-catenin,Bcl-2 were significantly higher than those in the propofol group(P<0.05).Conclusion:Propofol anesthesia can induce cognitive function and hippocampal neuron apoptosis in ra
作者
苏孟勤
SU Mengqin(Surgical Anesthesia Department, Henan Provincial Hospital, Henan 450019, China)
出处
《淮海医药》
CAS
2020年第6期551-553,557,共4页
Journal of Huaihai Medicine
