摘要
目的:检测胆管细胞癌中微小RNA-194-5p(miR-194-5p)和上皮型钙黏蛋白(E-cadherin)的表达,分析其临床意义及相关性。方法:选择胆管细胞癌49例作为观察组,选择正常胆管上皮组织49例作为对照组。应用实时荧光定量PCR法(qPCR)检测两组中miR-194-5p的表达,应用免疫组化法检测观察组中E-cadherin和神经型钙黏蛋白(N-cadherin)的表达。建立胆管癌细胞系的空白对照组、空载体转染组和miR-194-5p转染组,采用Western Blot法检测E-cadherin和N-cadherin的表达。结果:胆管细胞癌中miR-194-5p的表达明显低于对照组,miR-194-5p在不同肿物最大径、神经累犯、分化程度、转移及脉管癌栓的分组中差异有统计学意义。生存分析显示miR-194-5p低表达者生存时间短。miR-194-5p与E-cadherin具有正相关性(r=0.68,P=0.014),miR-194-5p与N-cadherin具有负相关性(r=-0.62,P=0.013)。与空白对照组和空载体转染组相比,miR-194-5p转染组E-cadherin的表达升高,N-cadherin的表达下降。结论:miR-194-5p在胆管细胞癌中低表达,与病变发展及生物学行为有关。miR-194-5p与E-cadherin具有正向协同作用,对调节细胞黏附有一定价值。miR-194-5p可能是胆管细胞癌独立的预后因子。
Objective:To detect the expression of miRNA-194-5p(miR-194-5p)and E-cadherin in cholangiocarcinoma,explore the relationship between miR-194-5p and E-cadherin.Methods:49 cases of cholangiocarcinoma were selected as the observation group,and 49 cases of normal bile duct epithelial tissue were selected as the control group.Expression of miR-194-5p was detected by real-time fluorescent quantitative PCR(qPCR)in the two groups.Expression of E-cadherin and N-cadherin were detected by immunohistochemistry in the observation group.We established blank control group,empty vector group and miR-194-5p transfection group of cholangiocarcinoma cell line.Expression of E-cadherin and N-cadherin were detected by Western Blot methods.Results:Expression of miR-194-5p was significantly lower in observation group than that in the control group.Expression of miR-194-5p was statistically significant in maximum diameter,nerve involvement,differentiation degree,metastasis and vascular tumor thrombus.Low expression of miR-194-5p had a short survival time by survival analysis.Positive correlation was found between miR-194-5p and E-cadherin(r=0.68,P=0.014).Negative correlaton was found between miR-194-5p and N-cadherin(r=-0.62,P=0.013).The expression of E-cadherin was increased in miR-194-5p group than that blank control group and empty vector group.The expression of N-cadherin was decreased in miR-194-5p group than that blank control group and empty vector group.Conclusion:Lower expression of miR-194-5p was found in cholangiocarcinoma,which is related to the development and biological behavior.miR-194-5p has a targeted relationship with E-cadherin,which has a certain value in regulating cell adhere.miR-194-5p may be an independent prognostic factor for cholangiocarcinoma.
作者
赵臣
贾聿明
张江华
张克俭
王金
ZHAO Chen;JIA Yuming;ZHANG Jianghua;ZHANG Kejian;WANG Jin(General Surgery of the 81 st Military Hospital of the PLA,Hebei Zhangjiakou 075000,China;Hepatobiliary Surgery,the Fourth Hospital of Hebei Medical University,Hebei Shijiazhuang 050000,China)
出处
《现代肿瘤医学》
CAS
北大核心
2021年第4期632-636,共5页
Journal of Modern Oncology
基金
张家口市重点研发计划项目(编号:1921124H)。
