摘要
目的建立密云地区腹泻患者致泻大肠埃希氏菌的毒力基因及耐药基因数据库。方法采集腹泻患者粪便样本,通过实时荧光定量聚合酶链式反应(polymerase chain reaction,PCR)方法鉴定致泻大肠埃希氏菌并确定毒力基因,采用微量肉汤稀释法确定耐药性,采用普通PCR方法对耐药基因进行检测。结果 2112份粪便样本中检出致泻大肠埃希氏菌114株,肠聚集性大肠埃希氏菌(enteroaggregative Escherichia coli,EAEC)占57.9%,毒力基因以astA+pic、eae为主,萘啶酸(nalidixic acid, NAL)、氨苄西林(ampicillin, AMP)、四环素(tetracycline, TET)、磺胺异恶唑(sulfamethoxazole, SUL)耐药率均超过30%, 41株菌株多重耐药,主要流行的耐药因子为GyrA、GyrB、blaTEM、ParC、aadA1、tetA、tetB、SUL2、floR、aac(3′)-IIa。结论密云地区腹泻患者致泻大肠埃希氏菌多重耐药性及耐药基因的存在情况较为普遍,应开展长期的主动监测。
Objective To establish the database of virulence genes and drug resistance genes of Escherichia coli causing diarrhea in Miyun area. Methods Fecal samples were collected from patients with diarrhea, and the virulence genes of Escherichia coli were identified by real-time fluorescent quantitative polymerase chain reaction(PCR), drug resistance was determined by broth microdilution method, and drug resistance genes were detected by ordinary PCR method. Results Totally 114 strains of diarrhoeal Escherichia coli were detected in 2112 fecal samples, and 57.9% of them were enteroaggregative Escherichia coli(EAEC). The main virulence genes were astA+pic and eae. The drug resistance rates of nalidixic acid(NAL), ampicillin(AMP), tetracycline(TET) and sulfamethoxazole(SUL) were more than 30%. The 41 strains were multi resistant, and the main resistant factors were GyrA, GyrB, blaTEM, ParC, aad A1, tetA, tet B, SUL2, floR and aac(3′)-IIa. Conclusion The multi drug resistance and drug resistance genes of pathogenic Escherichia coli causing diarrhea in Miyun area are common, and long-term active monitoring should be carried out.
作者
张巍巍
冯宝立
王丽丽
ZHANG Wei-Wei;FENG Bao-Li;WANG Li-Li(Miyun District Center for Disease Control and Prevention,Beijing 101500,China;Beijing Center for Disease Control and Prevention,Beijing 100013,China)
出处
《食品安全质量检测学报》
CAS
北大核心
2021年第2期751-756,共6页
Journal of Food Safety and Quality
基金
科技部“食品安全关键技术研发”重点专项项目(2017YFC1601400)。
关键词
致泻大肠埃希氏菌
耐药性
耐药基因
diarrheogenic Escherichia coli
drug resistance
drug resistance gene
