摘要
【目的】研究棕点石斑鱼(Epinephelus tukula,♀)与蓝身大斑石斑鱼(Epinephelus fuscoguttatus,♂)杂交F1代(下称"金虎斑")的染色体核型。【方法】采用头肾-PHA注射法制备金虎斑染色体,并分析其核型。【结果与结论】金虎斑二倍体染色体数为48,包括23对端部着丝粒染色体,1对亚中部着丝粒染色体,核型公式是2n=48=2sm+46t,染色体臂数(NF)为50。与其他石斑鱼核型相比,金虎斑染色体核型特点符合典型的高位类群鱼类核型特征;根据染色体臂数,金虎斑属于特化类群。
【Objective】To study the karyotype of the hybrids(named Jinhu grouper)of Epinephelus fuscoguttatus(♀)×Epinephelus tukula(♂).【Method】The head kidney-PHA injection method was used to prepare the chromosomes of the Jinhu grouper,and their karyotypes were analyzed.【Result and Conclusion】The number of diploid chromosomes of Jinhu grouper was 48,including 23 pairs of telocentric chromosomes,1 pair of sub-centromere chromosomes,the karyotype formula is 2n=48=2sm+46t,the number of chromosome arms(NF)was 50.Compared with the karyotypes of other groupers,the karyotype characteristics of Jinhu grouper showed the typical pattern of the high group of fish evolution taxonomy.According to the number of chromosome arms,Jinhu grouper belong to the specialization group.
作者
吴玉萍
田永胜
李振通
张晶晶
黎琳琳
王林娜
李子奇
陈帅
牛艺彭
翟介明
WU Yu-ping;TIAN Yong-sheng;LI Zhen-tong;ZHANG Jing-jing;LI Lin-lin;WANG Lin-na;LI Zi-qi;CHEN Shuai;NIU Yi-peng;ZHAI Jie-ming(Key Laboratory of Sustainable Development of Marine Fisheries,Ministry of Agriculture,Yellow Sea Fisheries Research Institute,Chinese Academy of Fishery Sciences,Qingdao 266071,China;Laboratory for Marine Fisheries Science and Food Production Processes,Qingdao National Laboratory for Marine Science and Technology,Qingdao 266000,China;College of Fisheries and Life Science,Dalian Ocean University,Dalian 116023,China;College of Fisheres and Life Sciences,Shanghai Ocean University,Shanghai 201306,China;Chinese Academy of Agricultural Sciences,Beijing 100081,China;Ming Bo Aquatic Co.Ltd.,Laizhou 261400,China)
出处
《广东海洋大学学报》
CAS
北大核心
2021年第1期119-123,共5页
Journal of Guangdong Ocean University
基金
山东省重点研发计划(2019GHY112063)
山东省良种工程(2019LZGC020)
烟台市高端人才引进“双百计划”项目
中国水产科学研究院基本科研业务费(2020XT0601)
中国水产科学研究院黄海水产研究所基本科研业务费(20603022019002,20603022020015)。
