摘要
【目的】创建高油酸(high oleic,HO)油菜新种质,探明HO新种质中高油酸性状的遗传模式,明确HO新种质油酸含量变化的生理特性,为培育HO油菜新品种奠定基础。【方法】采用辐射处理油菜萌动发芽种子,获得初级诱变群体后在后续世代利用极端选择法结合小孢子培养技术筛选油菜HO新种质。以HO新种质分别与3个不同遗传背景的常规油菜品系为亲本组合杂交构建6个世代(P1、P2、F1、BC1P1、BC1P2和F2)的遗传群体,测定各群体脂肪酸含量后应用主基因+多基因混合遗传模型联合分析方法对遗传群体的高油酸性状进行遗传分析。测定HO新种质种子发芽过程中子叶、不同温度处理下苗期营养器官以及角果成熟过程中种子的油酸含量,明确其变化规律及生理效应。【结果】通过辐射获得油酸含量显著变化的初级诱变群体,在后续世代持续利用极端选择法得到平均油酸含量升高20个百分点的高世代群体,采用小孢子培养得到纯合稳定的油菜HO双单倍体群体,最终根据品质性状筛选成功得到HO新种质B161,其油脂中油酸含量为85%,亚麻酸含量为3%。以B161为HO亲本和常规品系杂交配置得到3个具有不同遗传背景的遗传群体并测定获得各群体的油酸含量表型数据。脂肪酸含量相关性分析表明,十八碳脂肪酸中油酸含量与亚油酸含量和亚麻酸含量具有显著负相关关系。遗传分析结果表明,该种质中高油酸性状由2对具有加性效应的主效基因控制,并且2对基因对油酸含量的效应值接近。生理分析表明,常温下HO品系营养器官(根、茎、叶和叶柄)的油酸含量均显著高于常规品系,亚麻酸含量显著低于常规品系。低温下HO品系营养器官的油酸含量降低,但仍高于常规品系;常规品系油酸含量在低温下稳定。低温下两类品系营养器官的亚麻酸含量均显著提高,但HO品系亚麻酸含量仍低于常规品系。在种
【Objective】This study is to create the new high oleic(HO)canola germplasm,to explore its genetic mode and the physiological characters of the HO trait,which will lay a foundation for breeding HO canola varieties.【Method】The primary mutation population with was obtained by radiation treatment of germinating canola seeds.The new HO germplasm was screened by extreme selection method combined with microspore culture technology in subsequent generations.The genetic populations of six generations(P1,P2,F1,BC1P1,BC1P2 and F2)were constructed by crossing the HO Germplasm with three conventional canola lines with different genetic background.After the fatty acid content of each population was determined,the genetic analysis of high oleic acid content in the genetic population was analyzed by the mixed major-gene plus polygene inheritance model.The oleic acid content in cotyledons during seed germination,vegetative organs at seedling stage in different temperature regimes and seeds during silique ripening process of the HO germplasm were detected to explore their change patterns and physiological effects.【Result】The primary mutation population with significantly increased oleic acid content was obtained by radiation treatment,and then the high generation population with 20-percent-increased oleic acid content was obtained by using extreme selection method in subsequent generations and the double haploid population was obtained by microspore culture.Finally,a new HO germplasm B161(C18:1=85%,C18:3=3%)was successfully screened according to the quality traits.Three genetic populations with different genetic background were obtained by crossing B161 as HO parent with three other conventional lines.The correlation analysis of fatty acid contents showed that there was a significant negative correlation between oleic acid content,linoleic acid content and linolenic acid content.The results of genetic analysis showed that the high oleic acid content was controlled by two major genes with additive effect,and their effect
作者
龙卫华
浦惠明
高建芹
胡茂龙
张洁夫
陈松
LONG WeiHua;PU HuiMing;GAO JianQin;HU MaoLong;ZHANG JieFu;CHEN Song(Institute of the Industrial Crops,Jiangsu Academy of Agriculture Sciences/Key Lab of Cotton and Rapeseed(Nanjing)of Ministry of Agriculture,Nanjing 210014)
出处
《中国农业科学》
CAS
CSCD
北大核心
2021年第2期261-270,共10页
Scientia Agricultura Sinica
基金
国家重点研发项目(2016YFD0101300)
江苏省现代作物生产协同创新中心(JCIC-MCP)。
关键词
油菜
辐射诱变
种质创新
高油酸性状
遗传分析
低温响应
Brassica napus L.
radiation mutagenesis
germplasm creation
high oleic trait
genetic analysis
response to low temperature
