摘要
目的探讨虚拟导航超声气管镜(VBN)灌洗液miRNA检测对周围型肺癌的诊断价值。方法纳入2017年6月~2019年6月河北中石油中心医院呼吸与危重症医学科收治的56例周围型肺癌患者设为疾病组,选取56例肺部良性病变患者设为对照组。均行VBN灌洗液miRNA检测,比较两组灌洗液中miRNA-21、miRNA-155和miRNA-205相对表达量,分析疾病组不同病理特征患者灌洗液中miRNA-21、miRNA-155和miRNA-205相对表达量,分析VBN灌洗液miRNA检测对周围型肺癌的诊断价值。结果与对照组比较,疾病组VBN灌洗液中miRNA-21、miRNA-155和miRNA-205相对表达量明显升高,差异有统计学意义(P<0.05);疾病组组织学分类为腺癌、临床分期为Ⅲ-Ⅳ期、分化程度为低分化患者的灌洗液中miRNA-21、miRNA-155和miRNA-205相对表达量相较同类其它明显升高,差异有统计学意义(P<0.05);ROC曲线分析提示VBN灌洗液中miRNA-21、miRNA-155和miRNA-205检测诊断周围性肺癌的曲线下面积分别为0.988、0.862、0.831,其中miRNA-21诊断周围性肺癌的灵敏度、特异度最高;相关性分析提示VBN灌洗液中miRNA相对表达量与周围性肺癌的组织学分类(腺癌)、临床分期呈明显正相关,与分化程度呈明显负相关(P<0.05)。结论VBN灌洗液中miRNA-21、miRNA-155和miRNA-205检测对周围型肺癌的诊断价值高,其中miRNA-21诊断周围型肺癌的灵敏度、特异度最高,三者的检测利于周围型肺癌组织学分类、临床分期及分化程度的鉴别诊断,可为患者早期诊治提供重要参考。
Objective To explore diagnostic value of miRNA detection in virtual bronchoscopic navigation(VBN)lavage fluid for peripheral lung cancer.Methods 56 patients with peripheral lung cancer(disease group)and 56 patients with benign lung lesions(control group)who were treated in the department of respiratory and critical care medicine at CNPC Central hospital from June 2017 to June 2019 were selected as the study subjects.All of them completed VBN lavage fluid miRNA detection.The relative expression quantities of miRNA-21,miRNA-155 and miRNA-205 in lavage fluid were compared between the two groups.The relative expression quantities of miRNA-21,miRNA-155 and miRNA-205 in lavage fluid of patients with different pathological features in disease group were analyzed.The diagnostic value of miRNA detection in VBN lavage fluid for peripheral lung cancer was analyzed.Results Compared with control group,relative expression quantities of miRNA-21,miRNA-155 and miRNA-205 in VBN lavage fluid were significantly higher in disease group(P<0.05).The relative expression quantities of miRNA-21,miRNA-155 and miRNA-205 in lavage fluid were significantly higher in patients with adenocarcinoma as histological typing,clinical staging at stageⅢ-Ⅳand poor differentiation than the other groups(P<0.05).ROC curve analysis indicated that areas under the curve(AUC)of miRNA-21,miRNA-155 and miRNA-205 in VBN lavage fluid for diagnosis of peripheral lung cancer were 0.988,0.862 and 0.831,respectively.The sensitivity and specificity of miRNA-21 were the highest in diagnosis of peripheral lung cancer.Correlation analysis indicated that relative expression quantities of miRNA in VBN lavage fluid were significantly positively correlated with histological type(adenocarcinoma)and clinical stage of peripheral lung cancer,while significantly negatively correlated with differentiation degree(P<0.05).Conclusion The detection of miRNA 21,miRNA 155 and miRNA 205 in VBN lavage fluid has high diagnostic value for peripheral lung cancer.The sensitivity and speci
作者
颜晶晶
张业
刘颖
刘政
蔡青波
YAN Jingjing;ZHANG Ye;LIU Ying;LIU Zheng;CAI Qingbo(Department of Respiratory and Critical Care Medicine, CNPC Central Hospital, Langfang 065000, Hebei, China;Department of Infection, Langfang People's Hospital, Langfang 065000, Hebei, China)
出处
《西部医学》
2021年第1期79-83,共5页
Medical Journal of West China
基金
廊坊市科学技术研究与发展计划自筹经费项目(2017013046)。
关键词
周围型肺癌
虚拟导航超声气管镜
灌洗液
早期诊断
Peripheral lung cancer
Virtual bronchoscopic navigation
Bronchoscope lavage fluid
Diagnosis
