摘要
为评价不同沙棘品种(系)间抗寒性差异,以201307、201308、201319、晚黄、无刺丰、芬兰、TF2-31、杂54、巨人、深秋红10个不同沙棘亚种的一年生枝条为试验材料,首先通过田间寒害症状调查观测不同材料间抗寒性大致顺序,再通过实验室模拟低温胁迫,对丙二醛含量、脯氨酸含量、超氧化物歧化酶(SOD)活性等生理指标检测,印证田间寒害症状的表现顺序,最后通过植株相对电导率换算半致死温度(LT50)及相关指标的数值变化研究不同沙棘品种(系)间抗寒性差异。结果表明:相对电导率与脯氨酸含量随温度的降低而升高,SOD活性呈现先升高后降低,丙二醛(MDA)浓度随温度降低呈现先降低后升高,不同生理指标与沙棘抗寒性测定结果一致,结合半致死温度(LT50)及田间抗寒性调查结果综合评价,得出10个沙棘不同品种(系)抗寒性存在明显差异,芬兰品种抗寒性最好,其次为杂54,抗寒性最差的为俄罗斯大果沙棘品种201307和201308,其抗寒性顺序为芬兰>杂54>无刺丰>晚黄>2012-11>201319>巨人>深秋红>201307>201308。
In order to evaluate the difference of cold resistance among different Seabuckthorn varieties(lines),the annual branches of 10 different subspecies of Hippophae rhamnoides subspecies 201307,201308,201319,Wanhuang,Wucifeng,Fenlan,TF2-31,Za 54,Juren and Shenqiuhong were used as experimental materials.The cold resistance order of different materials was observed through field investigation of cold injury symptoms.Proline content,superoxide enzyme(SOD)activity and other physiological indicators were detected to confirm the performance order of cold injury symptoms in the field.Finally,the differences of cold resistance among different seabuckthorn varieties(lines)were studied through the conversion of plant relative conductivity into half lethal temperature(LT50)and the numerical changes of related indexes.The results showed that the relative conductivity and proline content increased with the decrease of temperature,the activity of SOD increased first and then decreased with the decrease of temperature,and the concentration of malondialdehyde(MDA)decreased first and then increased with the decrease of temperature.Different physiological indexes had the same results on the cold resistance of Hippophae rhamnoides.Combined with the semi lethal temperature(LT50)and the field cold resistance investigation results,the cold resistance of 10 seabuckthorn varieties(lines)was obvious different.The order of cold resistance was Fenlan>Za 54>Wucifeng>Wanhuang>2012-11>201319>Juren>Shenqiuhong>201307>201308.
作者
唐克
单金友
吴雨蹊
周双
房磊
王蕊
王肖洋
TANG Ke;SHAN Jin-you;WU Yu-xi;ZHOU Shuang;FANG Lei;WANG Rui;WANG Xiao-yang(Institute of Rural Revitalization Science and Technology,Heilongjiang Academy of Agricultural Sciences,Harbin 150028,China;Suiling Agricultural Technology Extension Center,Suiling 152200,China)
出处
《黑龙江农业科学》
2021年第1期91-95,共5页
Heilongjiang Agricultural Sciences
基金
黑龙江省农业科学院院级科研项目(2018YYYF032)
国家水利部沙棘开发管理中心项目(2018-zg-kj-004)
黑龙江省农业科学院“农业科技创新跨越工程”专项“果树优异种质资源创新及关键栽培技术研究”项目(HNK2019CX11)。
关键词
沙棘
品种(品系)
抗寒性
Hippophae rhamnoides
seabuckthorn
varieties
cold resistance
