摘要
目的:探讨良性前列腺增生(BPH)患者前列腺组织与外周血辅助性T细胞(Th)1/Th2/Th17相关细胞因子的表达及临床意义。方法:选取120例接受外科手术治疗的BPH患者为BPH组,采集术中前列腺组织标本,根据病理检查是否伴有前列腺炎症(PI)分为单纯BPH组和BPH-PI组。检测前列腺组织中相关细胞因子蛋白表达,术前检测外周血Th1/Th2/Th17细胞比例和IL-17A、IL-2、TNF-α、IFN-γ、IL-4、IL-6、IL-10水平,并选取40例健康男性作为对照组。结果:BPH组患者Th1细胞数、Th1/Th2比值和Th17细胞数较对照组明显升高(P<0.05),且BPH-PI组显著高于单纯BPH组(P<0.05)。BPH组外周血IL-17A、IL-2、TNF-α、IFN-γ、IL-4、IL-6、IL-10水平均显著高于对照组(P<0.05),且BPH-PI组显著高于单纯BPH组(P<0.05)。BPH-PI组患者前列腺组织中IL-17A、IL-2、TNF-α、IFN-γ、IL-4、IL-6、IL-10蛋白表达显著高于单纯BPH组(P<0.05)。结论:BPH患者存在Th1/Th2/Th17相关细胞因子表达紊乱,且合并PI时更为显著。Th2优势分化和Th17过表达与BPH的发生发展显著相关。
Objective:To investigate expression of helper T cell(Th)1/Th2/Th17 and related cytokines in prostate and peripheral blood of patients with benign prostatic hyperplasia(BPH)and in prostate tissues its clinical significance.Methods:120 patients with BPH who underwent surgical treatment were enrolled as BPH group.Intraoperative prostate tissues specimens were collected,and were divided into simple BPH group and BPH-PI group according to whether there was prostate inflammation(PI)based on pathological check results.Expressions of related cytokines proteins were detected,percentage of Th1/Th2/Th17 cells and IL-17A,IL-2,TNF-α,IFN-γ,IL-4,IL-6 and IL-10 levels in peripheral blood were detected,before surgery 40 healthy men were selected as control group.Results:Frequency of Th1 cells,ratio of Th1/Th2 and frequency of Th17 cells in BPH group were significantly increased than control group(P<0.05),and BPH-PI group were significantly higher than simple BPH group(P<0.05).Peripheral blood levels of IL-17A,IL-2,TNF-α,IFN-γ,IL-4,IL-6,IL-10 in BPH group were significantly higher than those in control group(P<0.05),and BPH-PI group was significantly higher than simple BPH group(P<0.05).Expressions of IL-17A,IL-2,TNF-α,IFN-γ,IL-4,IL-6 and IL-10 in prostate tissues in BPH-PI group were significantly higher than those in simple BPH group(P<0.05).Conclusion:Expressions of Th1/Th2/Th17 cytokines is abnormal in patients with BPH,and it is more obvious when complicated with PI.Dominant differentiation of Th2 and Th17 overexpression is significantly correlated with occurrence and development of BPH.
作者
张力杰
陈明(指导)
朱伟东
ZHANG Li-Jie;CHEN Ming;ZHU Wei-Dong(Department of Urology,Zhongda Hospital Affiliated to Southeast University,Nanjing 210009,China)
出处
《中国免疫学杂志》
CAS
CSCD
北大核心
2020年第24期3030-3035,共6页
Chinese Journal of Immunology
