摘要
目的通过研究炎性细胞因子IL-10、TNF-α在前列腺增生及前列腺癌组织中表达的差异,了解前列腺不同性质疾病的细胞生物学及免疫学特性。方法手术治疗前列腺组织增生以及前列腺癌各20例,均经病理学检查证实,并取正常男性前列腺组织做对照。利用免疫组织化学染色的方法,分别观察IL-10、TNF-α在所取标本各组织细胞中的表达,并利用图像分析技术比较各组标本光吸收密度的差异。结果 IL-10在3组标本组织细胞中都有表达,但以前列腺组织增生表达最强,前列腺癌次之,正常对照最弱,IL-10在上述3种组织中的表达分别为0.3121±0.0936、0.2958±0.0329、0.0816±0.0732;TNF-α在3组标本组织细胞中同样都有表达,以前列腺组织增生表达最强,前列腺癌次之,正常对照最弱,TNF-α在上述3种组织中的表达分别为0.4021±0.0652、0.3259±0.0487、0.1016±0.0825。选用方差分析Q检验进行分析,IL-10、TNF-α在3组标本的表达差异有统计学意义(P<0.05)。结论 IL-10、TNF-α在前列腺组织增生中都有较高表达;TNF-α在前列腺组织增生中较高的表达,可能与前列腺组织增生、组织结构破坏,从而引发的炎性反应有关;而IL-10在前列腺组织增生中较高的表达,可能是继发于炎性反应,并与临床症状有关;IL-10、TNF-α在前列腺癌组织细胞中都有表达。IL-10、TNF-α在前列腺癌组织细胞中的表达,可能与癌组织快速增长引发的炎性反应有关。
Objective To investigate the cytobiology in different prostate disease, and therefore to provide new screening and treatment meathods by analyzing the expression level of the inflammatory cell factors IL-10, TNF-α in prostate hyperplasia and prostate cancer tissues. Methods 20 cases of benign prostate hyperplasia and 20 cases prostate cancer were entered in this study respectively; all specimens were confirmed by pathological examinations. 1 case of normal prostate specimen was used as control. Immunohistochemical staining were performed to observe the expression of IL-10 and TNF-α, and the image analysis technique was used to compare the light absorption density of each specimens. Results IL-10 was expressed in three groups: benign prostate hyperplasia, prostate cancer and the normal control. Light absorption density value of IL-10 in the above three kinds of samples were 0.312 1±0.093 6、0.295 8±0.032 9、0.081 6±0.073 2. TNF-α in the above three groups were 0.402 1±0.065 2、0.325 9±0.048 7、0.101 6±0.082 5. The expression levels of IL-10 and TNF-α in three groups were different statisticantly. Conclusions The higher expression TNF-α in hyperplasia prostate tissue may due to prostate hyperplasia, structure damage, and inflammation. The higher expression of IL-10 in prostate hyperplasia, may result from inflammatory response. The expressions of IL-10, TNF-α in prostate cancer tissue maybe involved in inflammatory response caused by rapid growth of cancer tissue.
出处
《泌尿外科杂志（电子版）》
2012年第1期25-28,10,共5页
Journal of Urology for Clinicians(Electronic Version)
