摘要
为阐明文山市石漠化土壤真菌多样性情况及优势关键类群,采集石漠化样区中植物根际土壤,提取土壤总DNA,利用Illumina HiSeq平台对真核微生物ITS(Internal Transcribed Spacer)区扩增进行高通量测序后注释鉴定,分析了土壤真菌组成及多样性。结果表明,典型样本共获得168677条有效优化序列,平均长度为452.7 bp;α多样性指数显示3个样本间的真核微生物丰度与群落多样性差异不显著(P>0.05);检测到的序列聚类共得到314个OTUs,属于真菌的有7门,其中子囊菌门是占比最高的一门优势菌,其相对丰度最高值为71.07%,从纲阶元层看,座囊菌纲是占比最高的一属优势菌,其相对丰度最高值为37.96%;在目阶元层看格孢腔目是占比最高的一目优势菌,范围在20.67%~21.71%、其次是煤炱目(14.29%~15.66%)和肉座菌目(19.24%~20.38%);从属水平上看未分类真核微生物达52.70%~53.98%,其次是Boeremia(15.10%~16.88%)和芽枝霉属13.25%~14.28%)、旋孢腔菌属(3.66%~6.12%)、丝核菌属(2.70%~3.24%)、伞菌属(2.53%~2.87%)、球腔菌属(1.04%~1.43%)组成了关键优势真菌类群;样本中仅鉴定出球囊霉目的AM真菌,占比较低为1.05%~1.09%。Heatmap热图和主成分分析(PCA)显示样本群落结构组间差异较大。
To illustrate the diversity of soil fungal in rocky desertification area of Wenshan city and find out the predominant groups,the samples are collected from rhizosphere soil of plants in rocky desertification-like areas,soil total DNA is extracted,and paper uses the Illumina HiSeq platform on ITS eukaryotic microbes(Internal Transcribed Spacer)to determine after the high-throughput sequencing identification of area augmentation,and analyzes the composition of soil fungi and diversity.Results show that:a total of 168677 effective and majorized sequences from the soils typical samples are obtained,the average length is 452.7bp.Theαdiversities index indicate undiffered significantly in three samples(P>0.05).A total of 314 OTUs are detected,belonging to 7 fungi plylas,among which Ascomycota of dominant microflora account for a relatively high proportion which is up to 69.23~71.07%.At class level,the dominant microflora,Dothideomycetes and Sordariomycetes,account for a relatively high proportion,with relative abundance of 36.67~37.96%and 20.22~21.50%,respectively.At order level,the dominant microflora are Platyophryid(20.67~21.71%),Capnodiales(14.29~15.66%)and Hypocreales(19.24~20.38%).At generic level,the unclassified eukaryotic microorganisms are up to 52.70~53.98%,followed by Boeremia(15.10~16.88%),Cladosporium(13.25~14.28%),Cochliobolus(3.66~6.12%),Rhizoctonia(2.70~3.24%),Agaricus(2.53~2.87%)and Mycosphaerella(1.04~1.43%).In the samples,only AM fungi of Glomerales were identified,accounting for 1.05~1.09%.The Heatmap and principal component analysis(PCA)shows that there are significant differences in the sample community structure between groups.
作者
沈清清
赵芳
胡彦
吴风志
胡国贤
SHEN Qingqing;ZHAO Fang;HU Yan;WU Fengzhi;HU Guoxian(School of Environment and Resource,Wenshan University,Wenshan Yunnan 663099,China;School of Chemical Engineering,Wenshan University,Wenshan Yunnan 663099,China)
出处
《文山学院学报》
2020年第6期23-29,共7页
Journal of Wenshan University
基金
云南省教育厅科学研究基金项目“文山市石漠化土壤丛枝菌根真菌多样性研究”(2014Y473)
云南省地方本科高校基础研究联合专项“云南文山喀斯特石漠化区域从枝菌根真菌多样性与土壤因子的相关性研究”(2018FH001028)
文山学院本科教学质量与教学改革工程项目“‘微生物学’一流本科课程”(WYZL200304)
文山学院本科教学质量与教学改革工程项目“‘微生物学’重点课程”建设(WYZL180202)。
关键词
石漠化
土壤真菌
多样性
群落结构
高通量测序
rocky desertification
soil fungi
diversity
community structure
high throughput sequencing
