摘要
目的:观察中风1号方对急性脑出血(acute intracerebral hemorrhage,AICH)模型大鼠的影响,探讨其初步机制。方法:采用自体尾部血液注入法复制大鼠急性脑出血模型。将大鼠随机分为假手术组、模型组、中风1号方低剂量(6.75 g·kg^-1)组和中风1号方高剂量(13.5 g·kg^-1)组,连续给予相应的药物灌胃3天。用Longa 5级评分法评估神经功能损伤程度;计算脑组织含水量;检测脑组织伊文思蓝(evans blue,EB)含量,评价血脑屏障通透性;ELISA法检测脑出血周围组织肿瘤坏死因子α(tumor necrosis factor,TNF-α)、白细胞介素1β(interleukin-1β,IL-1β)水平。结果:与假手术组比较,模型组大鼠24 h、72 h的Longa 5级评分,脑组织含水量,EB含量,TNF-α、IL-1β水平均升高(P<0.05,P<0.01)。与模型组比较,中风1号方低剂量可降低模型大鼠神经行为学评分(72 h),脑组织含水量(72 h),EB含量,TNF-α、IL-1β水平(72 h)(P<0.05,P<0.01);中风1号方高剂量组可降低模型大鼠的神经行为学评分(72 h),脑组织含水量(72 h),EB含量(24 h、72 h),TNF-α、IL-1β水平(24 h、72 h)(P<0.01)。结论:中风1号方能改善急性脑出血模型大鼠神经功能损伤,减轻脑水肿,缓解血脑屏障受损,其机制可能与降低脑组织TNF-α、IL-1β水平有关。
Objective:To observe the effect of Zhongfeng No.1 Prescription on acute intracerebral hemorrhage(AICH)model rats and explore its preliminary mechanism.Methods:The model of acute cerebral hemorrhage in rats was replicated by autologous tail blood injection.The rats were randomly divided into sham operation group,model group,Zhongfeng No.1 Prescription low-dose(6.75 g·kg^-1)group and Zhongfeng No.1 Prescription high-dose(13.5 g·kg^-1)group,and given corresponding drugs continuously 3 days.The Longa 5-level scores method was used to evaluate the degree of neurological damage;calculate the water content of brain tissue;detect the content of evans blue(EB)in brain tissue to evaluate the permeability of the blood-brain barrier;the ELISA method was used to detect the levels of tumor necrosis factor-α(TNF-α)and interleukin-1β(IL-1β)in tissues surrounding cerebral hemorrhage.Results:Compared with the sham operation group,the Longa 5-level scores,brain tissue water content,EB content,TNF-α,IL-1βlevels of the model group increased at 24 h and 72 h(P<0.05,P<0.01);compared with the model group,the low dose of Zhongfeng No.1 prescription can reduce the water content of brain tissue(72 h),EB content,TNF-α,IL-1βlevels(72 h)(P<0.05,P<0.01);the high-dose group of Zhongfeng No.1 prescription can reduce the neurological function score(72 h),brain tissue water content(72 h),EB content(24 h,72 h),TNF-α,IL of model rats-1βlevel(24 h,72 h)(P<0.01).Conclusion:Zhongfeng No.1 Prescription can improve the nerve function damage of acute cerebral hemorrhage model rats,reduce brain edema,and relieve blood-brain barrier damage.The mechanism may be related to the reduction of brain tissue TNF-αand IL-1βlevels.
作者
邢锡熙
吴绍钦
沈存思
李建香
王益俊
严晶
XING Xixi;WU Shaoqin;SHEN Cunsi;LI Jianxiang;WANG Junyi;YAN Jing(Nanjing Jiangning District Hospital of Chinese Medicine,Nanjing Jiangsu China 211100;Institute of Pediatrics of Chinese Medicine,First Clinical Medical College,Nanjing University of Chinese Medicine/Jiangsu Provincial Key Laboratory of Children Respiratory Diseases(Chinese Medicine),Nanjing Jiangsu China 210023;Nanjing Hospital of Chinese Medicine Affiliated to Nanjing University of Chinese Medicine,Nanjing Jiangsu China 210001;Key Laboratory for Metabolic Diseases in Chinese Medicine,The First Clinical Medical College,Nanjing University of Chinese Medicine,Nanjing Jiangsu China 210023)
出处
《中医学报》
CAS
2021年第1期138-142,共5页
Acta Chinese Medicine
基金
国家自然科学基金项目(81704017、81904029)
南京市科技计划项目(201715072)。
关键词
急性脑出血
中风1号方
神经功能缺损
脑水肿
血脑屏障
肿瘤坏死因子α
白细胞介素1Β
acute cerebral hemorrhage
Zhongfeng No.1 Prescription
nerve function defect
brain edema
blood brain barrier
tumor necrosis factor(TNF-α)
interleukin-1β(IL-1β)
