摘要
红孢囊藻(Rhodosorus sp.)可以积累高含量多糖,在医药保健和生物能源领域具有潜在开发价值。为进一步评估红孢囊藻(Rhodosorus sp.)产业化潜力,研究了一株红孢囊藻SCSIO-45730在盐度不同的4种培养基中生长及总糖和β-葡聚糖积累特性,结果表明:改良的ASW培养基相对于其他培养基(f/2、Conway和Erdschreiber)更有利于红孢囊藻SCSIO-45730生物量和总糖的积累。以ASW培养基为基础培养基,研究该藻盐度适应性,结果显示:该藻可在18‰-58‰盐度范围内正常生长,随着盐度增加,藻细胞体积逐渐增大且出现聚团现象;在盐度为38‰时,获得最大生物量、总糖含量和产率以及β-葡聚糖产率,分别为13.70 g/L、48.52%DW、412.58 mg/L·d和119.70 mg/L·d,β-葡聚糖含量随着盐度增加而降低;该藻在户外平板光生物反应器中总糖和β-葡聚糖单位体积产量分别为0.73 g/L和0.23 g/L。结果表明,红孢囊藻SCSIO-45730具有较广的盐度适应性、高产总糖和β-葡聚糖潜力,是生产生物乙醇和β-葡聚糖的理想原料。
Rhodosorus sp.has the potential to be used in medicine,healthcare and bioenergy due to its capacity of accumulating high carbohydrates content.In order to evaluate its industrial potential,the growth,total carbohydrates andβ-glucans accumulation of this strain in 4 different culture media with different salinities were investigated.Results showed that the modified ASW medium was more beneficial for the biomass and total carbohydrates accumulation in Rhodosorus sp.SCSIO-45730 compared to other media(f/2,Conway and Erdschreiber).The salinity adaptability of this alga was studied using ASW medium as the basic medium.Results indicated that this alga grew normally in the salinity of 18‰-58‰.The cell size gradually increased and the algal cell aggregated with the increase of salinity.The highest biomass,total carbohydrates content and productivity,andβ-glucans productivity of Rhodosorus sp.SCSIO-45730 were obtained at the salinity of 38‰,which was 13.70 g/L,48.52%DW,412.58 mg/L·d and 119.70 mg/L·d,respectively,while theβ-glucans content decreased with the increase of salinity.The total carbohydrates andβ-glucans volumetric yield of this alga in outdoor flat-plate photobioreactor were 0.73 g/L and 0.23 g/L,respectively.In conclusion,Rhodosorus sp.SCSIO-45730 is capable of adaptation to a wide range of salinity and has high potential for total carbohydrates andβ-glucans production.It is considered as an optimal feedstock for the production of bioethanol andβ-glucans.
作者
代禄梅
李涛
吴华莲
吴后波
向文洲
DAI Lu-mei;LI Tao;WU Hua-lian;WU Hou-bo;XIANG Wen-zhou(Key Laboratory of Tropical Marine Bio-resources and Ecology,South China Sea Institute of Oceanology,Chinese Academy of Sciences,Guangzhou 510301;University of Chinese Academy of Sciences,Beijing 100049;Southern Marine Science and Engineering Guangdong Laboratory(Guangzhou),Guangzhou 511458;Institute of South China Sea Ecology and Environmental Engineering,Chinese Academy of Sciences,Guangzhou 510301)
出处
《生物技术通报》
CAS
CSCD
北大核心
2021年第1期205-214,共10页
Biotechnology Bulletin
基金
广东省省级科技计划项目(2019B030316027)
北海市“十三五”海洋经济创新发展示范项目(Bhsfs004)
南方海洋科学与工程广东省实验室(广州)人才团队引进重大专项(GML2019ZD0406)
中国科学院重点部署项目(ZDRW-ZS-2017-2-2)
广东省新能源和可再生能源研究开发与应用开放基金(E039kf0301)。
关键词
红孢囊藻
盐度
总糖
生物乙醇
Β-葡聚糖
Rhodosorus
salinity
total carbohydrates
bioethanol
β-glucans
