摘要
以6个月生的石榴‘泰山红’的实生苗和扦插苗为材料,分析不同浓度NaCl对根和叶片中活性氧的积累、膜质过氧化程度和抗氧化酶活性的影响。结果表明:随着盐浓度的增加,石榴苗盐害加重,单株生物量下降,O2·-产生速率和丙二醛含量逐渐增加;超氧化物歧化酶、过氧化氢酶、过氧化物酶、抗坏血酸过氧化物酶活性以及谷胱甘肽和可溶性蛋白含量升高。在盐胁迫下,活性氧在石榴的根和叶片中大量积累,细胞膜脂过氧化加剧,生长受到抑制,抗氧化酶、谷胱甘肽和可溶性蛋白能够一定程度的缓解氧化毒害,而且扦插苗的耐盐性比实生苗强。开展‘泰山红’石榴的耐盐性研究,旨在为盐碱地区选育提供依据。
Based on the 6-month-old Punica granatum ’Taishanhong’ seedlings and cuttings, the effects of different concentrations of NaCl on the accumulation of reactive oxygen in root and leaves, the degree of membrane peroxidation and antioxidant enzyme activity were studied. The results showed that with the increase of salinity, the salt damage increased, the biomass per plant decreased, the O2·-production rate and the content of malondialdehyde(MDA) gradually increased;the activities of superoxide dismutase(SOD), catalase(CAT), peroxidase(POD), ascorbate peroxidase(APX) and the content of glutathione(GSH) and soluble proteins increased.Research shows that under salt stress, reactive oxygen in the root and leaves of P. granatum accumulated, and cell membrane lipid peroxidation intensified, growth inhibited. Antioxidant enzymes, GSH and soluble proteins can alleviate oxidation toxicity to a certain degree, and the salt tolerance of cuttings better than seedlings. The research on salt tolerance of P. granatum ’Taishanhong’ was conducted to provide basis for selection and breeding in saline-alkali regions.
作者
董建梅
刘翠玉
赵玉洁
苑兆和
Dong Jianmei;Liu Cuiyu;Zhao Yujie;Yuan Zhaohe(Co-Innovation Center for Sustainable Forestry in Southern China,Nanjing Forestry University,Nanjing Jiangsu 210037,China;College of Forestry,Nanjing Forestry University,Nanjing Jiangsu 210037,China)
出处
《西南林业大学学报（自然科学）》
CAS
北大核心
2021年第1期47-54,共8页
Journal of Southwest Forestry University：Natural Sciences
基金
南京林业大学高层次人才科研启动资金(GXL2014070)资助
江苏省高校优势学科建设工程项目(PAPD)资助。
关键词
石榴
盐胁迫
活性氧
抗氧化酶
生长特性
扦插苗
实生苗
Punica granatum
salt stress
reactive oxygen
antioxidant enzyme
growth characteristics
cutting seedling
seedling
