摘要
目的分析肺移植术后稳定状态受者1年内T淋巴细胞亚群的动态变化及影响因素。方法收集行同种异体肺移植手术且术后处于稳定状态的41例受者的临床资料。采用流式细胞术检测受者术前、术后2周及每个月(术后1年内)外周血T淋巴细胞亚群绝对值和比值。分析受者年龄、性别、体质量指数(BMI)、手术方式、原发性移植物功能障碍(PGD)发生情况及原发病对T淋巴细胞绝对值的影响。结果肺移植术后1年内,CD3^+、CD3^+CD4^+、CD3^+CD8^+T淋巴细胞绝对值及CD4^+/CD8^+比值随时间变化差异有统计学意义(均为P<0.001)。与术前相比,术后12个月CD3^+、CD3^+CD4^+T淋巴细胞绝对值差异均无统计学意义(P=0.659、0.109),CD3^+CD8^+T淋巴细胞绝对值升高(P=0.02),而CD4^+/CD8^+比值下降,差异有统计学意义(P<0.001)。肺移植受者的年龄、性别、BMI、手术方式以及术后是否出现PGD对CD3^+CD4^+、CD3^+CD8^+T淋巴细胞绝对值的动态变化无影响(P>0.05)。肺移植术前原发病对CD3^+CD4^+T淋巴细胞的变化无影响,但感染性肺疾病受者术后CD3^+CD8^+T淋巴细胞绝对值较高(P<0.05)。结论肺移植术后稳定状态受者CD3^+、CD3^+CD4^+、CD3^+CD8^+T淋巴细胞绝对值在术后早期较低,随后逐渐恢复,术后6个月后达到稳态。其动态变化与受者年龄、性别、BMI、手术方式以及术后是否出现PGD无关。
Objective To analyze the dynamic changes and the influencing factors of T lymphocyte subsets in recipients with stable graft status within 1 year after lung transplantation.Methods Clinical data of 41 recipients with stable graft status after allogeneic lung transplantation were analyzed.The absolute value and ratio of T lymphocyte subsets in peripheral blood from recipients were measured by flow cytometry before operation,2 weeks and each month(within 1 year)after operation,respectively.The effects of age,gender,body mass index(BMI),surgical method,incidence of primary graft dysfunction(PGD)after operation,and primary disease upon the absolute values of T lymphocytes were evaluated.Results Within 1 year after lung transplantation,the absolute values of CD3^+,CD3^+CD4^+,CD3^+CD8^+T lymphocytes and CD4^+/CD8^+ratio were changed over time(all P<0.001).Compared with preoperative values,there was no statistical significance in the absolute values of CD3^+and CD3^+CD4^+T lymphocytes at 12 months after operation(P=0.659,0.109),whereas the absolute value of CD3^+CD8^+T lymphocytes was increased(P=0.02)and the CD4^+/CD8^+ratio was decreased(P<0.001).Age,gender,BMI,surgical method and incidence of PGD after operation exerted no significant effect on the dynamic changes of absolute values of CD3^+CD4^+and CD3^+CD8^+T lymphocytes(all P>0.05).Primary disease before lung transplantation exerted no effect on the changes of CD3^+CD4^+T lymphocytes,whereas the postoperative absolute value of CD3^+CD8^+T lymphocytes was higher in recipients with infectious lung diseases(P<0.05).Conclusions The absolute values of CD3^+,CD3^+CD4^+,CD3^+CD8^+T lymphocytes in recipients with stable graft status after lung transplantation are relatively low in the early stage after lung transplantation,then gradually restore,and stabilize at 6 months after operation.Dynamic changes are not associated with age,gender,BMI,surgical method and incidence of PGD after operation of recipients.
作者
练巧燕
陈奥
徐鑫
韦兵
蔡宇航
黄丹霞
何建行
巨春蓉
Lian Qiaoyan;Chen Ao;Xu Xin;Wei Bing;Cai Yuhang;Huang Danxia;He Jianxing;Ju Chunrong(Guangzhou Institute of Respiratory Health,State Key Laboratory of Respiratory Disease,the First Affiliated Hospital of Guangzhou Medical University,Guangzhou 510012,China)
出处
《器官移植》
CAS
CSCD
北大核心
2021年第1期83-89,共7页
Organ Transplantation
基金
国家重点实验室青年自主课题(SKLRD-QN-201710)
广东省自然科学基金(2018A030313107)
广州医科大学附属第一医院临床研究中心临床自主探索项目(2019GIRHZ04)。
