摘要
目的利用二代测序技术筛选侵袭性垂体腺瘤组织中差异表达的lncRNA,分析其在侵袭性垂体腺瘤发病机制中可能发挥的潜在作用。方法通过RNA测序分析4例侵袭性垂体腺瘤患者和3例非侵袭性垂体腺瘤患者的lncRNA和mRNA的表达情况,筛选出差异表达的lncRNA和mRNA。据此,构建lncRNA-mRNA共表达网络,并进行GO和KEGG通路分析。结果对侵袭性和非侵袭性垂体瘤的lncRNA和mRNA差异表达分析,共获得35个差异表达的lncRNA和463个差异表达的mRNA。共表达网络显示FAM66A、TPTEP1、AC017116.11、RP11-1114A5.4和RP11-246K15.1在网络中处于关键位置。结论 lncRNA-mRNA共表达网络的建立,发现一些差异表达的lncRNA可能在侵袭性垂体腺瘤的发病机制中发挥关键作用,可能成为侵袭性垂体腺瘤新的诊断标志物和治疗靶点。
Objective To screen differentially expressed lncRNAs in invasive pituitary adenomas by secondgeneration sequencing,and to explore their potential role in the pathogenesis of invasive pituitary adenomas.Methods The expressions of lncRNA and mRNA in 4 patients with invasive pituitary adenoma and 3 patients with non-invasive pituitary adenoma were analyzed by RNA sequencing to single out the differentially expressed lncRNA and mRNA.Accordingly,lncrNA-mRNA co-expression networks were constructed and GO and KEGG pathways were analyzed.Results A total of 35 differentially expressed lncRNAs and 463 differentially expressed mRNAs were obtained by analyzing the differentially expressed lncRNAs and mRNAs in both invasive and non-invasive pituitary tumors.The co-expression network showed that FAM66A,TPTEP1,AC017116.11,RP11-1114A5.4 and RP11-246K15.1 were in a key position in the network.Conclusion Through the establishment of coexpression network of lncRNA-mRNA,we found that some new lncRNA may play a key role in the pathogenesis of invasive pituitary adenomas and may be used as novel biomarkers of invasive pituitary adenomas.
作者
蒋国庆
邓兴力
李晓娟
钱源
JIANG Guo-qing;DENG Xing-li;LI Xiao-juan;QIAN Yuan(Dept.of Obstetrics,The 1st Affiliated Hospital of Kunming Medical University;Dept.of Neurosurgery,The 1st Affiliated Hospital of Kunming Medical University;Dept.of Clinical Laboratory,The 1st Affiliated Hospital of Kunming Medical University;Yunnan Provincial Key laboratory of Laboratory Medicine;Yunnan Innovation Team of Clinical Laboratory and Diagnosis,Kunming Yunnan 650032,China)
出处
《昆明医科大学学报》
CAS
2020年第12期42-48,共7页
Journal of Kunming Medical University
基金
国家自然科学基金资助项目(81760273)
云南省卫生科技计划基金资助项目(2018NS0112)
省部共建云南生物资源保护与利用国家重点实验室开放课题计划基金资助项目(2018KF006)
昆明市科技计划基金资助项目(2019-1-S-25318000001501)。
