摘要
【背景】伪狂犬病毒(pseudorabies virus,PRV)是养猪生产中的一类重要病原,自2011年以来,我国接种了Bartha-K61疫苗的养殖场暴发了大规模的伪狂犬病疫情。【目的】调查目前四川省PRV的流行病学以及毒株的遗传进化,对2018-2019年从86个猪场收集的384份疑似PRV感染样本进行病原学检测。【方法】根据PRV-g E基因检测引物对采集的384份样品进行PCR扩增,并对不同季节、不同地区的PRV阳性率进行统计,将PRV感染与临床症状的相关性进行统计学分析。选择部分PRV阳性样本在BHK-21细胞上进行病毒的分离,随后进行分离毒株的g C、g E、TK基因的遗传进化分析。【结果】PRV阳性猪只的比率为9.9%(38/384);阳性猪场比率为16.3%(14/86);流产母猪的PRV阳性率为32.1%(27/84);种公猪阳性率为2.0%(4/198);神经症状猪的PRV阳性率为11.4%(4/35);呼吸症状猪的PRV阳性率为4.5%(3/67)。统计学分析表明,PRV感染与母猪和公猪繁殖障碍症状相关(P<0.01)。其中,冬季(12月、1月、2月)PRV阳性率最高,约为33.0%(31/94);春季(3月、4月、5月)的阳性率为9.1%(3/33);夏季和秋季的阳性率分别约为1.5%(2/130)和1.6%(2/127)。在2018-2019年共分离出3株PRV毒株,分别命名为PRV-SN、PRV-DJY、PRV-CD。PRV-XJ为本实验室2016年在四川省分离的一株毒株,为了了解毒株的遗传进化信息,先后扩增了这4个毒株的g C、g E、TK基因。序列比对表明四川分离株与国内株相似,存在额外的零星性突变和缺失。【结论】养殖场仍应加强对种猪群中伪狂犬病的净化。
[Background]Pseudorabies virus(PRV)is an important pathogen to pig industry,a large-scale of pseudorabies(PR)has swept many Bartha-K61-vaccined farms in China since 2011.[Objective]In order to investigate the epidemiological characteristics of PRV strains prevalent in Sichuan province,China,384 suspected PRV infected samples were collected from 86 pig farms in 2018-2019.[Methods]According to the amplification of PRV-g E gene,384 samples were detected by PCR.The positive rates of PRV in different seasons and regions were counted,and the correlation between PRV infections and clinical symptoms was statistically analyzed.We selected some PRV positive samples for virus isolation through BHK-21 cells,followed by g C,g E and TK genetic informatic analysis of isolated strains.[Results]The individual positive rate of PRV was 9.9%(38/384);the group positive rate of PRV was 16.3%(14/86);the positive rate in aborted fetuses was 32.1%(27/84);the positive rate in semen of breeding boars was 2.0%(4/198);the positive rate in nervous symptoms pig was 11.4%(4/35);the positive rate in respiratory symptoms pig was 4.5%(3/67).Statistical analysis indicated that PRV infection was associated with pig reproductive failure(P<0.01).The highest PRV infection rate was detected in winter(December,January,February),with a positive rate of 33.0%(31/94);Spring(April,March,May)ranked the second highest positive rate of 9.1%(3/33);Summer and autumn was about 1.5%(2/130)and 1.6%(2/127)respectively.Totally,we isolated 3 PRV strains between 2018-2019,named PRV-SN,PRV-DJY,PRV-CD respectively.PRV-XJ,a strain isolated by our laboratory in 2016 in Sichuan province was also included,these strains were used for g C,g E,TK gene sequencing.Sequence alignment shows that Sichuan isolates were similar to those Chinese isolates,except for sporadic mutations and deletions.[Conclusion]Farms should strengthen the purification of pseudorabies in breeding herds.
作者
殷鑫欢
鲁令华
王进疆
谢勇
朱玲
周莉媛
陈弟诗
徐志文
YIN Xin-Huan;LU Ling-Hua;WANG Jin-Jiang;XIE Yong;ZHU Ling;ZHOU Li-Yuan;CHEN Di-Shi;XU Zhi-Wen(Animal Biotechnology Center,College of Veterinary Medicine,Sichuan Agricultural University,Chengdu,Sichuan 611130,China)
出处
《微生物学通报》
CAS
CSCD
北大核心
2020年第12期4142-4154,共13页
Microbiology China
基金
国家重点研发计划(2018YFD0500802,2018YFD051102)
国家农业产业技术体系四川兽药创新团队专项(CARS-SVDIP)
四川省科技厅应用基础研究项目(2019YJ0560)。
关键词
伪狂犬病毒
基因分析
流行病学调查
Pseudorabies virus
Genetic analysis
Epidemiological investigation
