摘要
目的:观察复方芪麻胶囊对多种实验性高血压大鼠的降血压作用,以及对相关血管活性因子的调节作用。方法:开展对自发性高血压大鼠(spontaneously hypertensive rats,SHR)血压动态变化及降钙素基因相关肽(calcitonin gene-related peptide,CGRP)、一氧化氮(nitric oxide,NO)、神经肽Y(neuropeptide Y,NPY)含量影响;对左旋硝基精氨酸诱导高血压大鼠模型血压及NO含量影响;对丙酸睾丸素诱导内分泌型高血压大鼠血压及睾酮(testosterone,T)、血管紧张素Ⅱ(angiotensin II,AngⅡ)、醛固酮(aldosterone,Ald)含量影响等实验,检测复方芪麻胶囊连续灌胃给药后大鼠的血压变化和CGRP,NO,NPY,T,AngⅡ,Ald等含量的变化。结果:复方芪麻胶囊明显降低SHR大鼠清醒状态下的收缩压、舒张压,随着服药时间延长其降血压作用呈现逐渐降低逐步平稳的趋势,同时升高CGRP和NO含量(P<0.05),降低NPY水平(P<0.05);降低左旋硝基精氨酸诱导高血压大鼠血压数值(P<0.01),提高NO含量(P<0.01或P<0.05);降低丙酸睾丸素诱导内分泌型高血压大鼠模型血压数值(P<0.01),降低AngII和Ald含量(P<0.05或P<0.01)。结论:复方芪麻胶囊对多种实验性高血压大鼠模型具有显著的降血压作用,其机制可能与改善内皮细胞功能,升高CGRP和NO含量,降低NPY水平,抑制血管内AngⅡ和Ald的分泌和改善体内水钠潴留有关。
Objective: This study aims to observe the hypotensive effect of compound Qima capsule on various experimental hypertensive rats and the regulation of related vasoactive factors.Methods: The effect of compound Qima capsule on dynamic changes of blood pressure and the levels of calcitonin gene-related peptide(CGRP),nitric oxide(NO) and neuropeptide Y(NPY) in spontaneously hypertensive rats(SHR),the blood pressure and NO concentration in hypertensive rats induced by L-nitroarginine,as well as the blood pressure and the levels of testosterone(T),angiotensin Ⅱ(AngⅡ) and aldosterone(Ald) in endogenous hypertensive rats induced by testosterone propionate were observed.The changes of blood pressure and the levels of CGRP,NO,NPY,T,Ang Ⅱ and Ald in rats after continuous intragastric administration of compound Qima capsule were detected.Results: Compound Qima capsule significantly reduced the systolic and diastolic blood pressures of SHR rats in conscious state.With the prolonging of medication time,its hypotensive effect gradually decreased and steadily stabilized.At the same time,it increased the contents of CGRP and NO(P<0.05),decreased the level of NPY(P<0.05);reduced the blood pressure of hypertensive rats induced by L-nitroarginine(P<0.01),increased NO content(P<0.01 or P<0.05);reduced blood pressure values in endogenous hypertensive rats induced by testosterone propionate(P<0.01),and decreased the contents of AngⅡ and Ald(P<0.05 or P<0.01).Conclusion:Compound Qima capsule had significant hypotensive effect on various experimental hypertensive rat models.The mechanism was probably related to improvement of endothelial cell function,increasing the contents of CGRP and NO,decreasing NPY levels,inhibiting the secretions of AngⅡ and Ald in blood vessels,and improving water and sodium retention in vivo.
作者
杨九妹
陈玉兴
甘海宁
黄雪君
蔡大可
黄丹娥
王清海
YANG Jiu-mei;CHEN Yu-xing;GAN Hai-ning;HUANG Xue-jun;CAI Da-ke;HUANG Dan-e;WANG Qing-hai(Fifth Clinical Medical School,Guangzhou University of Chinese Medicine,Guangzhou 510405,China;Guangdong Second Traditional Chinese Medicine Hospital,Guangdong Research Institute of Traditional Chinese Medicine Manufacturing Technology,Guangzhou 510095,China;Guangdong Provincial Key Laboratory of Research and Development in Traditional Chinese Medicine,Guangzhou 510095,China)
出处
《中国新药杂志》
CAS
CSCD
北大核心
2020年第23期2723-2728,共6页
Chinese Journal of New Drugs
基金
广东省科技计划项目(2017A070701017)
广东省中医药局重大科技项目(粤中医办函[2018]6号)。
关键词
复方芪麻胶囊
高血压
血管活性因子
compound Qima capsule
hypertension
vasoactive factors
