摘要
目的:观察补乌煎剂联合308准分子光治疗进展期白癜风的临床疗效,及对趋化因子配体(CXCL)10/趋化因子受体(CXCR)3信号轴募集皮肤T淋巴细胞的影响。方法:根据进展期白癜风患者的纳入标准选取70例患者,随机分为治疗组(35例)和对照组(35例)。治疗组采用补乌煎剂联合308准分子光疗,对照组单独予308准分子光疗。疗程结束后(3个月)比较2组临床疗效,采用酶联免疫吸附法(ELISA)检测外周血CXCL10水平,流式细胞仪检测外周血淋巴细胞中CXCR3^+CD4^+和CXCR3^+CD8^+T细胞的比例,并选取健康组10例进行比较。结果:临床疗效比较,治疗3个月后,治疗组疗效优于对照组(P<0.05);2组患者治疗后外周血CXCL10表达均明显下降,且治疗组优于对照组(P<0.05);2组患者治疗后外周血淋巴细胞中CXCR3^+CD4^+和CXCR3^+CD8^+T细胞比例下降,治疗组优于对照组(P<0.05)。结论:补乌煎剂联合308准分子光可控制进展期白癜风的发展和促进白斑的复色,其机制可能与阻断CXCL10/CXCR3信号轴,从而抑制CD8^+T细胞的细胞毒作用有关。
Objective:To observe the clinical efficacy of Buwu decoction combined with 308 excimer light in the treatment of advanced vitiligo,and its effect on the recruitment of skin T lymphocytes via the chemokine ligand(CXCL)10/chemokine receptor(CXCR)3 signal axis.Methods:According to the inclusion criteria,70 patients with advanced vitiligo were selected and randomly divided into the treatment group(35 cases)and the control group(35 cases).The treatment group was given Buwu decoction combined with 308 excimer phototherapy,and the control group was treated with 308 excimer phototherapy alone.After a 3-month treatment,the clinical effects of the two groups were compared.The level of CXCL10 in peripheral serum was de tected by ELISA.The proportion of CXCR3^+CD4^+and CXCR3^+CD8^+in T cells of peripheral blood lymphocytes was detected by flow cytometry.Results:After 3 months of treatment,the clinical outcome of the treatment group was better than that of the control group(P<0.05).The expression of CXCL10 in peripheral blood of the two groups decreased significantly after treatment,and the decrease in the treatment group was greater than the control group(P<0.05).The proportion of CXCR3^+CD4^+and CXCR3^+CD8^+in T cells of peripheral blood lymphocytes in the two groups decreased significantly after treatment,which was statistically significant compared with the control group before the treatment(P<0.05).Conclusion:Buwu decoction combined with308 excimer light could not only control the development of advanced vitiligo,but also promote repigmentation.The mechanism may be related to the block of the CXCL10/CXCR3 signal axis,thereby inhibiting the cytotoxicity of CD8^+T cells.
作者
章纬
张虹亚
刘涛峰
王建锋
曹宇
于庆生
ZHANG Wei;ZHANG Hong-ya;LIU Tao-feng;WANG Jian-feng;CAO Yu;YU Qing-sheng(College of Postgraduates,Anhui University of Chinese Medicine,Hefei 230031,China)
出处
《临床皮肤科杂志》
CAS
CSCD
北大核心
2020年第12期726-731,共6页
Journal of Clinical Dermatology
